NKX2.2 Antibody (883411) [Unconjugated]

Western blot shows lysates of A-673 human Ewing's sarcoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NKX2.2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NKX2.2 was detected in immersion fixed A673 human Ewing sarcoma cell line using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

NKX2.2 was detected in immersion fixed rat cortical stem cells differentiated for 7 days using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 883411
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NKX2.2 Antibody (883411) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NKX2.2
  Met1-Lys128
  Accession # O95096
• Specificity: Detects human NKX2.2 in ELISA and Western Blot.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX2.2 Antibody (883411) [Unconjugated]

• Homeobox protein NK-2 homolog B
• NK-2 (Drosophila) homolog B
• NK2 homeobox 2
• NK2 transcription factor related, locus 2 (Drosophila)
• NK2 transcription factor related, locus 2
• NK2 transcription factor-like protein B
• NKX2.2
• NKX2.2homeobox protein Nkx-2.2
• NKX2B
• NKX2BNK-2 homolog B

Background

NKX2.2, also known as NKX2-2 or NKX2B, is an approximately 30 kDa, 273 amino acid homeobox nuclear transcription factor of the NKX2 protein family. It specifies differentiation of pancreatic islet cells such as alpha, beta, PP, and epsilon lineages.  It also functions in developmental neuronal patterning of the ventral spinal cord in response to graded Sonic Hedgehog gradients and contributes pathfinding of commissural neurons. Within the region used as an immunogen, human NKX2.2 shares 96% and 97% amino acid sequence identity with mouse and rat NKX2.2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKX2.2 Antibody (MAB8167)(2)

Publications using MAB8167

• L Reiche, P Göttle, L Lane, P Duek, M Park, K Azim, J Schütte, A Manousi, J Schira-Hei, P Küry C21orf91 Regulates Oligodendroglial Precursor Cell Fate-A Switch in the Glial Lineage? Frontiers in Cellular Neuroscience, 2021-03-16;15(0):653075. 2021-03-16 [PMID: 33796011] (ICC, Rat)
• Integrator of Stress Responses Calmodulin Binding Transcription Activator 1 (Camta1) Regulates miR-212/miR-132 Expression and Insulin Secretion J Biol Chem, 2016-07-08;0(0):. 2016-07-08 [PMID: 27402838] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: NKX2-2
• Uniprot:
  • O95096(Human)