Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were ...read more
Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine, 0.15 M NaCl
0.05% Sodium Azide
Protein G purified
Alternate Names for ABCG8 Antibody (1B10A5)
ATP-binding cassette sub-family G member 8
ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
ATP-binding cassette, sub-family G (WHITE), member 8
GBD4ATP-binding cassette, subfamily G, member 8
Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ABCG8 Antibody (NBP1-71706). (Showing 1 - 3 of 3 FAQs).
I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (http://www.novusbio.com/ABCG8-Antibody_NBP1-83388.html); 2) Used for IHC-P in mouse intestine (species - mouse) (http://www.novusbio.com/ABCG8-Antibody-1B10A5_NBP1-71706SS.html); Out of the above two which do you and your team think will be more applicable for our purpose.
I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.
Please provide the ratio of the Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3.
The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3 is 0.05M Glycine, 0.5M Tris pH 7.4.
What is the concentration of Tris and Glycine in the buffer used for the ABCG8 antibody, NBP1-71706?
The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 is 0.05M Glycine, 0.5M Tris pH 7.4.
ABCG2: A Tumor Protector ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters... Read full blog post.
Exploring how ABCG8 Affects Heart Disease The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th... Read full blog post.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.