ABCG8 Antibody (1B10A5)


Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were more
Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
1 mg/ml

Order Details

ABCG8 Antibody (1B10A5) Summary

Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]
Membrane; Multi-pass membrane protein
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 25 ug
  • Knockout Validated
  • Western Blot 1:1000
Application Notes
This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.
Reviewed Applications
Read 1 Review rated 5
NBP1-71706 in the following applications:

Read Publications using
NBP1-71706 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    9 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for ABCG8 Antibody (1B10A5)

  • ATP-binding cassette sub-family G member 8
  • ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
  • ATP-binding cassette, sub-family G (WHITE), member 8
  • GBD4ATP-binding cassette, subfamily G, member 8
  • MGC142217
  • sterolin 2
  • sterolin-2
  • STSL


Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG8 Antibody (NBP1-71706)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-71706 Applications Species
Wang JQ, Li LL, Hu A Et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion Nature 2022-08-03 [PMID: 35922515] (WB)

Citation using the FITC version of this antibody.
Nyandwi JB, Ko YS, Jin H Et al. Rosmarinic Acid Exhibits a Lipid-Lowering Effect by Modulating the Expression of Reverse Cholesterol Transporters and Lipid Metabolism in High-Fat Diet-Fed Mice Biomolecules 2021-10-06 [PMID: 34680102] (WB, Mouse) WB Mouse
Li Zhiqiang, Kabir Inamul, Tietelman Gladys et al. Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function. Cell Death & Disease 2018-02-07 [PMID: 29415989] (WB, Mouse) WB Mouse
Xie Yan, Fung Ho Yee Joyce, Newberry Elizabeth P et al. Hepatic Mttp deletion reverses gallstone susceptibility in L-Fabp knockout mice. J Lipid Res. 2014-01-28 [PMID: 24474819] (Mouse) Mouse
von Kampen O, Buch S, Nothnagel M et al. Genetic and functional identification of the likely causative variant for cholesterol gallstone disease at the ABCG5/8 lithogenic locus. Hepatology 2012-08-16 [PMID: 22898925] (ICC/IF, Human) ICC/IF Human
Wang J, Grishin N, Kinch L, Cohen JC, Hobbs HH, Xie XS. Sequences in the nonconsensus nucleotide-binding domain of ABCG5/ABCG8 required for sterol transport. J Biol Chem;286(9):7308-14. 2011-03-04 [PMID: 21209088] (WB, Mouse) WB Mouse
Johnson BJ, Lee JY, Pickert A, Urbatsch IL. Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8 Biochemistry. 2010-04-27 [PMID: 20210363] (WB, Human) WB Human
Wang J, Zhang DW, Lei Y, Xu F, Cohen JC, Hobbs HH, Xie XS. Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. Biochemistry;47(18):5194-204. 2008-05-06 [PMID: 18402465] (WB, ICC/IF, IP, Mouse) WB, ICC/IF, IP Mouse
Zhang DW, Graf GA, Gerard RD, Cohen JC, Hobbs HH. Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8. J Biol Chem;281(7):4507-16. 2006-02-17 [PMID: 16352607] (WB, IP, Mouse) WB, IP Mouse
af GA, Yu L, Li WP, Gerard R, Tuma PL, Cohen JC, Hobbs HH. ABCG5 and ABCG8 are obligate heterodimers for protein trafficking and biliary cholesterol excretion. J Biol Chem;278(48):48275-82. 2003-11-28 [PMID: 14504269] (WB, Mouse, Human) WB Mouse, Human
Show All 11 Publications.

Review for ABCG8 Antibody (NBP1-71706) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71706:
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Images Ratings Applications Species Date Details
reviewed by:
Verified Customer
WB Human 12/24/2014


ApplicationWestern Blot
Sample TestedSee PMID 20210363

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG8 Antibody (NBP1-71706). (Showing 1 - 3 of 3 FAQs).

  1. I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (NBP1-83388); 2) Used for IHC-P in mouse intestine (species - mouse) (NBP1-71706SS); Out of the above two which do you and your team think will be more applicable for our purpose.
    • I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.
  2. Please provide the ratio of the Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3.
    • The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 lot A-3 is 0.05M Glycine, 0.5M Tris pH 7.4.
  3. What is the concentration of Tris and Glycine in the buffer used for the ABCG8 antibody, NBP1-71706?
    • The Tris-Glycine buffer used for the ABCG8 antibody, product NBP1-71706 is 0.05M Glycine, 0.5M Tris pH 7.4. 

Secondary Antibodies


Isotype Controls

Additional ABCG8 Products

Research Areas for ABCG8 Antibody (NBP1-71706)

Find related products by research area.

Blogs on ABCG8.

Understanding Sitosterolemia: How ABCG5-ABCG8 Dimer Affects Blood Sterol Levels
The ATP binding cassette (ABC) transporter family is the largest and most diverse family of membrane transport proteins and, as the name suggests, uses the energy generated by ATP hydrolysis to transport substrates across membranes. Eukaryotic ABC tra...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human


Gene Symbol ABCG8