ABCG8 Antibody (1B10A5)

Images

 
Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.
Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
1B10A5
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

ABCG8 Antibody (1B10A5) Summary

Immunogen
Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]
Localization
Membrane; Multi-pass membrane protein
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
ABCG8
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 25ug
Application Notes
This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-71706 in the following applications:

Publications
Read Publications using
NBP1-71706 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Mouse and human.

Alternate Names for ABCG8 Antibody (1B10A5)

  • ATP-binding cassette sub-family G member 8
  • ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
  • ATP-binding cassette, sub-family G (WHITE), member 8
  • GBD4ATP-binding cassette, subfamily G, member 8
  • MGC142217
  • sterolin 2
  • sterolin-2
  • STSL

Background

Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-23488
Species: Hu
Applications: ICC/IF (-), WB, ELISA, Flow, IHC, IHC-P
NB400-105
Species: Hu, Mu, Rt, Po, Ca, Ch, Ha, Md
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, GS
NB400-127
Species: Hu, Mu, Rt, Po
Applications: WB, EM, ICC/IF, IHC, IHC-P
NBP2-30887
Species: Hu
Applications: IHC, IHC-P
NBP1-89319
Species: Hu
Applications: WB, IHC, IHC-P
MAB9120
Species: Hu
Applications: ICC
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
NB400-157
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western
NB400-104
Species: Hu, Mu, Rt, ChHa, SyHa, Ha, Md, Pm, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB400-132
Species: Hu, Mu, Rt, ChHa, Ha, Mk, Rb
Applications: WB, ICC/IF, IHC, IHC-P, In vitro
NB400-156
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IP
NBP1-91996
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP1-42349
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-45981
Species: Hu
Applications: IHC
NBP1-54446
Species: Hu, Ch
Applications: WB, ELISA, GS, ICC/IF, IP
PP-A9033A-00
Species: Hu
Applications: WB, IHC, IP, DirELISA
NBP1-42348
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr
NBP1-71706
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for ABCG8 Antibody (NBP1-71706)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(2)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-71706 Applications Species
Xie Yan, Fung Ho Yee Joyce, Newberry Elizabeth P et al. Hepatic Mttp deletion reverses gallstone susceptibility in L-Fabp knockout mice. J Lipid Res. 2014 Jan 28 [PMID:24474819] (Mouse) Mouse
von Kampen O, Buch S, Nothnagel M et al. Genetic and functional identification of the likely causative variant for cholesterol gallstone disease at the ABCG5/8 lithogenic locus. Hepatology 2012 Aug 16 [PMID:22898925] (ICC/IF, Human) ICC/IF Human
Wang J, Grishin N, Kinch L, Cohen JC, Hobbs HH, Xie XS. Sequences in the nonconsensus nucleotide-binding domain of ABCG5/ABCG8 required for sterol transport. J Biol Chem;286(9):7308-14. 2011 Mar 4. [PMID:21209088] (WB, Mouse) WB Mouse
Johnson BJ, Lee JY, Pickert A, Urbatsch IL. Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8 Biochemistry. 2010 Apr 27 [PMID:20210363] (WB, Human) WB Human
Wang J, Zhang DW, Lei Y, Xu F, Cohen JC, Hobbs HH, Xie XS. Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. Biochemistry;47(18):5194-204. 2008 May 6. [PMID:18402465] (WB, IP, ICC/IF, Mouse) WB, IP, ICC/IF Mouse
Zhang DW, Graf GA, Gerard RD, Cohen JC, Hobbs HH. Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8. J Biol Chem;281(7):4507-16. 2006 Feb 17. [PMID:16352607] (WB, IP, Mouse) WB, IP Mouse
af GA, Yu L, Li WP, Gerard R, Tuma PL, Cohen JC, Hobbs HH. ABCG5 and ABCG8 are obligate heterodimers for protein trafficking and biliary cholesterol excretion. J Biol Chem;278(48):48275-82. 2003 Nov 28. [PMID:14504269] (WB, Human, Mouse) WB Human, Mouse

Reviews for ABCG8 Antibody (NBP1-71706) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71706:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/24/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20210363
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20210363

Details

Detection NotesSee PMID 20210363

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 20210363.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG8 Antibody (NBP1-71706). (Showing 1 - 1 of 1 FAQs).

  1. I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (http://www.novusbio.com/ABCG8-Antibody_NBP1-83388.html); 2) Used for IHC-P in mouse intestine (species - mouse) (http://www.novusbio.com/ABCG8-Antibody-1B10A5_NBP1-71706SS.html); Out of the above two which do you and your team think will be more applicable for our purpose.
    • I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71706AF405
Alexa Fluor 488 Labeled NBP1-71706AF488
Alexa Fluor 647 Labeled NBP1-71706AF647
Alexa Fluor 700 Labeled NBP1-71706AF700
Biotin Labeled NBP1-71706B
DyLight 405 Labeled NBP1-71706V
DyLight 488 Labeled NBP1-71706G
DyLight 550 Labeled NBP1-71706R
DyLight 650 Labeled NBP1-71706C
FITC Labeled NBP1-71706F
HRP Labeled NBP1-71706H

Additional ABCG8 Antibody (1B10A5) Products

Related Products by Gene

Bioinformatics Tool for ABCG8 Antibody (NBP1-71706)

Discover related pathways, diseases and genes to ABCG8 Antibody (NBP1-71706). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ABCG8 Antibody (NBP1-71706)

Discover more about diseases related to ABCG8 Antibody (NBP1-71706).
 

Pathways for ABCG8 Antibody (NBP1-71706)

View related products by pathway.

PTMs for ABCG8 Antibody (NBP1-71706)

Learn more about PTMs related to ABCG8 Antibody (NBP1-71706).
 

Research Areas for ABCG8 Antibody (NBP1-71706)

Find related products by research area.

Blogs on ABCG8.

Understanding Sitosterolemia: How ABCG5-ABCG8 Dimer Affects Blood Sterol Levels
The ATP binding cassette (ABC) transporter family is the largest and most diverse family of membrane transport proteins and, as the name suggests, uses the energy generated by ATP hydrolysis to transport substrates across membranes. Eukaryotic ABC tra...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol ABCG8
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-71706 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought