Niemann-Pick type C1 Like-1 Antibody [HRP]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

Niemann-Pick type C1 Like-1 Antibody [HRP] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 500-600). [Uniprot: Q6T3U3]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPC1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB400-128. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for Niemann-Pick type C1 Like-1 Antibody [HRP]

• Niemann-Pick C1-like protein 1
• NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• NPC11L1

Background

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Niemann-Pick type C1 Like-1 Antibody (NB400-128H)(1)

Publication using NB400-128H

• Levy E, Ouadda AB, Spahis S et al. PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells. Atherosclerosis 2013-02-04 [PMID: 23422832] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB400-128, catalog number NB400-128H.

Bioinformatics

• Gene Symbol: NPC1L1