NHERF-2 Antibody

Western Blot: NHERF-2 Antibody [NB100-2450] - (0.3ug/ml) Analysis of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450] - IHC analysis of NHERF-2 in paraffin embedded Human Kidney using NB100-2450 at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NHERF-2 Antibody Summary

• Immunogen: Peptide with sequence C-DGSAWKQDPFQ corresponding to internal region according to NP_001123484.1.
• Specificity: Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC9A3R2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in human kidney lysates (calculated MW of 37.4 kDa band according to BAA33216.1). IHC-P: Human kidney shows strong membranous staining in glomerulus-.
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NHERF-2 Antibody

• E3KARP
• Na(+)/H(+) exchange regulatory cofactor NHE-RF2
• NHE3RF2
• NHERF2MGC104639
• NHERF-2NHE3 kinase A regulatory protein E3KARP
• OCTS2
• SIP-1SRY-interacting protein 1
• sodium/hydrogen exchanger
• Sodium-hydrogen exchanger regulatory factor 2
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
• Solute carrier family 9 isoform A3 regulatory factor 2
• TKA-1SIP1
• Tyrosine kinase activator protein 1

Background

Gene ID(s): 9351 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NHERF-2 Antibody (NB100-2450)(1)

Publication using NB100-2450

• Cha B, Kim JH, Hut H et al. cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein. J Biol Chem 2005-04-29 [PMID: 15722341]

Bioinformatics

• Gene Symbol: SLC9A3R2
• Entrez:
  • 9351(Human)
• Uniprot:
  • BAA33216.1(Human)