NFYA Antibody

Western Blot: NFYA Antibody [NBP2-19533] - NF-YA is essential for lincRNA-p21 regulation by mutant P53 but not wild-type p53. The binding of p53 to NF-YA in HN6, Cal27 and HN30 cells was determined by coimmunoprecipitation. *: P < 0.05; **: P < 0.01 Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-019-0993-3) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: NFYA Antibody [NBP2-19533] - NFYA antibody detects NFYA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYA stained by NFYA antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.

Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.

Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE gel, diluted at 1:3000.

Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:3000.

Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000.

Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NFYA Antibody Summary

• Immunogen: Full length human NFYA Recombinant protein.
• Localization: Nucleus
• Predicted Species: Porcine (97%), Bovine (97%), Rhesus Macaque (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFYA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for NFYA Antibody

• CAAT box DNA-binding protein subunit A
• CBF-A
• CBF-B
• CCAAT-binding transcription factor subunit B
• FLJ11236
• HAP2 CCAAT-binding protein
• HAP2
• NF-YACAAT-box DNA binding protein subunit A
• Nuclear transcription factor Y subunit A
• nuclear transcription factor Y subunit alpha
• nuclear transcription factor Y, alpha
• Transcription factor NF-Y, A subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NFYA Antibody (NBP2-19533)(1)

Publication using NBP2-19533

• Jin S, Yang X, Li J et al. p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma Mol. Cancer 2019-03-11 [PMID: 30857539] (WB, Human)

Bioinformatics

• Gene Symbol: NFYA