NFYA Antibody

Images

 
Western Blot: NFYA Antibody [NBP2-19533] - NF-YA is essential for lincRNA-p21 regulation by mutant P53 but not wild-type p53. The binding of p53 to NF-YA in HN6, Cal27 and HN30 cells was determined by ...read more
Immunocytochemistry/ Immunofluorescence: NFYA Antibody [NBP2-19533] - NFYA antibody detects NFYA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. ...read more
Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.
Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE gel, diluted at 1:3000.
Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:3000.
Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000.
Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: NFYA Antibody [NBP2-19533] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFYA Antibody Summary

Immunogen
Full length human NFYA Recombinant protein.
Localization
Nucleus
Predicted Species
Porcine (97%), Bovine (97%), Rhesus Macaque (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFYA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-19533 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NFYA Antibody

  • CAAT box DNA-binding protein subunit A
  • CBF-A
  • CBF-B
  • CCAAT-binding transcription factor subunit B
  • FLJ11236
  • HAP2 CCAAT-binding protein
  • HAP2
  • NF-YACAAT-box DNA binding protein subunit A
  • Nuclear transcription factor Y subunit A
  • nuclear transcription factor Y subunit alpha
  • nuclear transcription factor Y, alpha
  • Transcription factor NF-Y, A subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00010153-B01P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-19535
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP
H00004802-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, RNAi, S-ELISA
NB600-233
Species: Hu, Mu
Applications: WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, ChIP, KD
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P
DPSG10
Species: Hu
NB100-2593
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB110-74569
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56082
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP1-88871
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-81776
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-45731
Species: Hu, Ca, Pm, All-NA
Applications: WB, IHC, IHC-P
NBP2-21584
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-85729
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-47123
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-86872
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-44687
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NFYA Antibody (NBP2-19533)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for NFYA Antibody (NBP2-19533) (0)

There are no reviews for NFYA Antibody (NBP2-19533). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NFYA Antibody (NBP2-19533) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional NFYA Products

Bioinformatics Tool for NFYA Antibody (NBP2-19533)

Discover related pathways, diseases and genes to NFYA Antibody (NBP2-19533). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NFYA Antibody (NBP2-19533)

Discover more about diseases related to NFYA Antibody (NBP2-19533).
 

Pathways for NFYA Antibody (NBP2-19533)

View related products by pathway.

PTMs for NFYA Antibody (NBP2-19533)

Learn more about PTMs related to NFYA Antibody (NBP2-19533).
 

Research Areas for NFYA Antibody (NBP2-19533)

Find related products by research area.

Blogs on NFYA

There are no specific blogs for NFYA, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NFYA Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NFYA