NFYB Antibody

Images

 
Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: NFYB Antibody [NBP2-19535] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB protein stained by NFYB antibody diluted at 1:500. Red: Phalloidin, a ...read more
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Paraffin-embedded rat stomach. NFYB antibody diluted at 1:500.
Chromatin Immunoprecipitation (ChIP): NFYB Antibody [NBP2-19535] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was ...read more
Western Blot: NFYB Antibody [NBP2-19535] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: NFYB Antibody [NBP2-19535] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Paraffin-embedded mouse intestine. NFYB antibody diluted at 1:500.
Immunoprecipitation: NFYB Antibody [NBP2-19535] - 293T whole cell lysate/extract A : 40 ug whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC-P, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFYB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (99%), Bovine (98%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFYB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Chromatin Immunoprecipitation (ChIP)
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NFYB Antibody

  • CAAT box DNA-binding protein subunit B
  • CBF-A
  • HAP3CBF-B
  • NF-YBCCAAT-binding transcription factor subunit A
  • Nuclear transcription factor Y subunit B
  • nuclear transcription factor Y subunit beta
  • nuclear transcription factor Y, beta
  • Transcription factor NF-Y, B subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NFYB
Uniprot