NFYB Antibody

Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NFYB Antibody [NBP2-19535] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB protein stained by NFYB antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Chromatin Immunoprecipitation (ChIP): NFYB Antibody [NBP2-19535] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.ChIP experiment and primer designs are based on Nucleic Acids Res. 2006;34(21):6272-85.

Western Blot: NFYB Antibody [NBP2-19535] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: NFYB Antibody [NBP2-19535] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Mouse intestine. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: NFYB Antibody [NBP2-19535] - 293T whole cell lysate/extract A : 40 ug whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 ug of NFYB antibody 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) as used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM, Xp
• Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NFYB Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Porcine (99%), Bovine (98%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFYB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Application Notes: Chip-Assay dependent
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for NFYB Antibody

• CAAT box DNA-binding protein subunit B
• CBF-A
• HAP3CBF-B
• NF-YBCCAAT-binding transcription factor subunit A
• Nuclear transcription factor Y subunit B
• nuclear transcription factor Y subunit beta
• nuclear transcription factor Y, beta
• Transcription factor NF-Y, B subunit

Background

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFYB
• Uniprot:
  • P25208(Human)