NFIL3 Antibody (714401) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8570) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 714401
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NFIL3 Antibody (714401) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NFIL3/E4BP4
  Lys140-Gly462
  Accession # Q16649
• Specificity: Detects human NFIL3/E4BP4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFIL3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFIL3 Antibody (714401) [Unconjugated]

• E4BP4
• E4BP4IL3BP1
• IL3BP1
• Interleukin-3 promoter transcriptional activator
• Interleukin-3-binding protein 1
• NFIL3
• NF-IL3A
• NFIL3AE4 promoter-binding protein 4
• nuclear factor interleukin 3 regulated protein
• nuclear factor interleukin-3-regulated protein
• nuclear factor, interleukin 3 regulated
• Transcriptional activator NF-IL3A

Background

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

1. Seillet C, et al (2014) J Exp Med. 211:1733.
2. Geiger TL, et al (2014) J Exp Med. 211:1723.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIL3
• Uniprot:
  • Q16649()