402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog #
FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog #
AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #
F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012)." class="big_lightbox">
402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog #
FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog #
AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #
F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012)." alt="Mouse splenocytes treated with 100 ng/mL Recombinant Mouse IL-2 (Catalog #
402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog #
FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog #
AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #
F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012)." class="big_thumb" />
FAB100P) and either (A) Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8570) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." alt="Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8570) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_thumb" />
402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." alt="Mouse splenocytes treated with 100 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_thumb" />
![Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.](http://images.novusbio.com/fullsize/NFIL3-Antibody-Western-Blot-NB100-842-img0003.jpg "Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.")
![Flow Cytometry: NFIL3 Antibody [NB100-842] - analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized](http://images.novusbio.com/fullsize/NFIL3-Antibody-Flow-Cytometry-NB100-842-img0004.jpg)
![SDS-Page: Recombinant Human NFIL3 Protein [H00004783-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004783-P01-1.jpg "SDS-Page: Recombinant Human NFIL3 Protein [H00004783-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: NFIL3 Antibody [NBP1-41077] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E4BP4 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E4BP4 was also immunoprecipitated by another rabbit anti-E4BP4 antibody, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NFIL3-Antibody-Western-Blot-NBP1-41077-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NFIL3 Antibody [NBP1-41077] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E4BP4 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E4BP4 was also immunoprecipitated by another rabbit anti-E4BP4 antibody, which recognizes an upstream epitope.")
![Western Blot: NFIL3 Overexpression Lysate (Adult Normal) [NBP2-04291] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFIL3.](http://images.novusbio.com/fullsize/NFIL3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04291-img0001.jpg "Western Blot: NFIL3 Overexpression Lysate (Adult Normal) [NBP2-04291] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFIL3.")
![Western Blot: NFIL3 Antibody [H00004783-B01P] - Analysis of NFIL3 expression in transfected 293T cell line by NFIL3 polyclonal antibody. Lane 1: NFIL3 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIL3-Antibody-Western-Blot-H00004783-B01P-img0001.jpg "Western Blot: NFIL3 Antibody [H00004783-B01P] - Analysis of NFIL3 expression in transfected 293T cell line by NFIL3 polyclonal antibody. Lane 1: NFIL3 transfected lysate(50.82 KDa). Lane 2: Non-transfected lysate.")
