NFIL3 Antibody (714401) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
714401
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

NFIL3 Antibody (714401) [Alexa Fluor® 647] Summary

Immunogen
E. coli-derived recombinant human NFIL3/E4BP4
Lys140-Gly462
Accession # Q16649
Specificity
Detects human NFIL3/E4BP4 in direct ELISAs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFIL3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFIL3 Antibody (714401) [Alexa Fluor® 647]

  • E4BP4
  • E4BP4IL3BP1
  • IL3BP1
  • Interleukin-3 promoter transcriptional activator
  • Interleukin-3-binding protein 1
  • NFIL3
  • NF-IL3A
  • NFIL3AE4 promoter-binding protein 4
  • nuclear factor interleukin 3 regulated protein
  • nuclear factor interleukin-3-regulated protein
  • nuclear factor, interleukin 3 regulated
  • Transcriptional activator NF-IL3A

Background

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.
  1. Seillet C, et al (2014) J Exp Med. 211:1733.
  2. Geiger TL, et al (2014) J Exp Med. 211:1723.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NFIL3
Uniprot