NFIL3 Antibody (714401) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: Flow
• Clone: 714401
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

NFIL3 Antibody (714401) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human NFIL3/E4BP4
  Lys140-Gly462
  Accession # Q16649
• Specificity: Detects human NFIL3/E4BP4 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFIL3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFIL3 Antibody (714401) [Alexa Fluor® 350]

• E4BP4
• E4BP4IL3BP1
• IL3BP1
• Interleukin-3 promoter transcriptional activator
• Interleukin-3-binding protein 1
• NFIL3
• NF-IL3A
• NFIL3AE4 promoter-binding protein 4
• nuclear factor interleukin 3 regulated protein
• nuclear factor interleukin-3-regulated protein
• nuclear factor, interleukin 3 regulated
• Transcriptional activator NF-IL3A

Background

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

1. Seillet C, et al (2014) J Exp Med. 211:1733.
2. Geiger TL, et al (2014) J Exp Med. 211:1723.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFIL3
• Uniprot:
  • Q16649()