HLF Antibody (M2)

Western Blot: HLF Antibody (M2) [H00003131-M04] - Western blot analysis of HLF over-expressed 293 cell line, cotransfected with HLF Validated Chimera RNAi or non-transfected control. Blot probed with H00003131-M04. GAPDH (36.1 kDa) used as loading control.

Immunocytochemistry/Immunofluorescence: HLF Antibody (M2) [H00003131-M04] - Immunofluorescence of to HLF ( Cat# H00003131-M04 ) on HeLa cell .

Western Blot: HLF Antibody (M2) [H00003131-M04] - analysis of HLF expression in transfected 293T cell line by HLF monoclonal antibody (M04), clone M2. Lane 1: HLF transfected lysate (33.2 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: M2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HLF Antibody (M2) Summary

• Immunogen: HLF (AAH36093, 1 a.a. ~ 295 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKMSRPLPLNPTFIPPPYGVLRSLLENPLKLPLHHEDAFSKDKDKEKKLDDESNSPTVPQSAFLGPTLWDKTLPYDGDTFQLEYMDLEEFLSENGIPPSPSQHDHSPHPPGLQPASSAAPSVMDLSSRASAPLHPGIPSPNCMQSPIRPGQLLPANRNTPSPIDPDTIQVPVGYEPDPADLALSSIPGQEMFDPRKRKFSEEELKPQPMIKKARKVFIPDDLKDDKYWARRRKNNMAAKRSRDARRLKENQIAIRASFLEKENSALRQEVADLRKELGKCKNILAKYEARHGPL
• Specificity: HLF - hepatic leukemia factor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLF
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HLF Antibody (M2)

• hepatic leukemia factor
• MGC33822

Background

This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLF Antibody (H00003131-M04)(1)

Publication using H00003131-M04

• Murakami Y, Higashi Y, Matsunaga N et al. Circadian clock-controlled intestinal expression of the multidrug resistance gene mdr1a in mice. Gastroenterology;135(5):1636-1644. 2008-11-01 [PMID: 18773899]

Bioinformatics

• Gene Symbol: HLF
• Entrez:
  • 3131(Human)
• OMIM:
  • 142385(Human)
• Uniprot:
  • AAH36093(Human)