NF-L Antibody - BSA Free

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-54669 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.

Western Blot: NF-L Antibody [NBP2-54669] - Analysis in control (vector only transfected HEK293T lysate) and NEFL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebral cortex shows strong positivity in neuronal processes.

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebellum shows strong positivity in neuronal processes in molecular layer.

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NF-L Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NF-L Antibody - BSA Free (NBP2-54669) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a Recombinant Protein corresponding to amino acids: SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRF
• Predicted Species: Mouse (97%), Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEFL
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NF-L Antibody - BSA Free

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEFL