NF-H Antibody

Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Imaging of Feline optic nerve at antibody dilution 1:5000. This image was submitted via customer Review.

Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217] - Analysis of a rat cerebellum section stained with NF-H antibody, dilution 1:5,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Western Blot: NF-H Antibody [NB300-217] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-Neurofilament Heavy antibody NB300-217.

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Rat neurons stained with 200kDa Neurofilament Heavy Antibody NB300-217.

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - SH-SY5Y cells stained with 200kDa Neurofilament Heavy Antibody NB300-217 (red) and Fibrillarin Antibody NB300-269 (green). Nuclear DNA is stained with Hoechst dye (blue).

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody NBP1-05217 [green] and Neurofilament Heavy (NF-H) antibody NB300-217 [red]. Blue is a DNA stain. NB300-217 binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NBP1-05217 which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.

Immunohistochemistry-Frozen: NF-H Antibody [NB300-217] - Canine optic nerve, antibody specifically labelled the axons (Green). Blue is DAPI. Image taken with an epifluorescent microscope and was incubated at 1:10000 for 1hr at RT.. Image from verified customer review.

Immunohistochemistry-Paraffin: NF-H Antibody [NB300-217] - NF-H immunoreactivity in a FFPE section of mouse trigeminal ganglion. Antibody was diluted 1 to 2000 and left on sections for 1h at room temperature. Image from verified customer review.

Immunohistochemistry-Frozen: Chicken Polyclonal NF-H Antibody [NB300-217] - Fixed frozen section of mouse optic nerve showing neurofilament heavy NB300-217 in green and S100B in red. NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of canine cecum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of feline colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of equine jejunum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of human colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
• Applications: WB, ELISA, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

NF-H Antibody Summary

• Immunogen: Native NF-H (phosphorylated) purified from bovine spinal cord.
• Marker: Neuronal Marker
• Specificity: Reacts very strongly with NF-H KSP type phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: NEFH
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20000
  • Immunohistochemistry Free-Floating 1:5000
  • Immunohistochemistry 1:20000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100000
• Theoretical MW: 200-220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Feline reactivity reported in verified customer review. Feline reactivity reported in scientific literature (PMID:33091431).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
• Preservative: 0.035% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for NF-H Antibody

• 200 kDa neurofilament protein
• KIAA0845
• NEFH
• Neurofilament Heavy (200kDa)
• neurofilament heavy polypeptide
• Neurofilament triplet H protein
• neurofilament, heavy polypeptide 200kDa
• neurofilament, heavy polypeptide
• NF200
• NFH
• NF-H

Background

Neurofilaments can be defined as the intermediate or 10nm filaments found specifically in neuronal cells. Neurofilaments appears as fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid axon impulse conduction. They are composed of a mixture of subunits which usually include the three neurofilament triplet proteins, known as Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-H Antibody (NB300-217)(11)

Publications using NB300-217

• Watson SS, Zomer A, Fournier N, Lourenco J et Al. Fibrotic response to anti-CSF-1R therapy potentiates glioblastoma recurrence Cancer Cell 2024-09-10 [PMID: 39255775]
• Jennifer Mecklenburg, Sergey A. Shein, Mostafa Malmir, Anahit H. Hovhannisyan, Korri Weldon, Yi Zou, Zhao Lai, Yu-Fang Jin, Shivani Ruparel, Alexei V. Tumanov, Armen N. Akopian Transcriptional profiles of non-neuronal and immune cells in mouse trigeminal ganglia Frontiers in Pain Research 2023-10-31 [PMID: 38028432]
• Runkel MT, Tarabishi A, Shay-Winkler K et al. The role of sympathetic innervation in neonatal muscle growth and neuromuscular contractures The FEBS journal 2023-07-18 [PMID: 37462535]
• Jagadeeshaprasad MG, Govindappa PK, Nelson AM et al. 4-Aminopyridine Induces Nerve Growth Factor to Improve Skin Wound Healing and Tissue Regeneration Biomedicines 2022-07-08 [PMID: 35884953]
• Shen M, Chen Z, Ming M, Cheng Z et Al. The acetylome of adult mouse sciatic nerve J Neurochem 2022-05-19 [PMID: 35585794]
• Kerkenberg N, Wachsmuth L, Zhang M Et al. Brain microstructural changes in mice persist in adulthood and are modulated by the palmitoyl acyltransferase ZDHHC7 The European journal of neuroscience 2021-08-06 [PMID: 34355442]
• Ho BL, Goh Q, Nikolaou S et al. NRG/ErbB signaling regulates neonatal muscle growth but not neuromuscular contractures in neonatal brachial plexus injury FEBS Letters 2021-03-01 [PMID: 33421114] (Immunohistochemistry-Frozen, Mouse)
• Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)
• Yamamoto H, Yamanashi Y, Takada T et al. Controlling the dose-dependent, synergistic and temporal effects of NGF and GDNF by encapsulation in PLGA microparticles for use in nerve guidance conduits for the repair of large peripheral nerve defects J Control Release 2019-05-02 [PMID: 31054993] (IHC-P, Rat)
• Thai TQ, Nguyen HB, Sui Y et al. Interactions between mitochondria and endoplasmic reticulum in demyelinated axons. Med Mol Morphol. 2018-11-17 [PMID: 30448927] (Mouse)
• Nikolaou S, Hu L, Cornwall R. Afferent Innervation, Muscle Spindles, and Contractures Following Neonatal Brachial Plexus Injury in a Mouse Model. J Hand Surg Am 2015-10-01 [PMID: 26319770] (IHC-Fr, Mouse)

Reviews for NF-H Antibody (NB300-217) (8)

Average Rating: 4.9

(Based on 8 reviews)

We have 8 reviews tested in 7 species: Human, Mouse, Canine, Cat, Dog, Feline, Horse.

reviewed by: Verified Customer IHC-P Human 04/18/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colon
• Species: Human
• Lot: 7575-8

Comments

• Comments: Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.

reviewed by: Verified Customer IHC-P Horse 04/18/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Jejunum
• Species: Horse
• Lot: 7575-8

Comments

• Comments: Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.

reviewed by: Verified Customer IHC-P Cat 04/18/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colon
• Species: Cat
• Lot: 7575-8

Comments

• Comments: Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.

reviewed by: Verified Customer IHC-P Dog 04/18/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Cecum
• Species: Dog
• Lot: 7578-8

Comments

• Comments: Sections were subject to heat induced epitope retrieval in Target Retrieval Solution for 20m in a vegetable steamer.

reviewed by: Verified Customer IHC-Fr Mouse 04/05/2024

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Optic Nerve
• Species: Mouse
• Lot: 7575-8

Comments

• Comments: NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488.

reviewed by: Kevin Thorburn IHC-P Mouse 04/06/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: FFPE
• Species: Mouse
• Lot: 7575-8

reviewed by: Verified Customer IHC-Fr Canine 02/06/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Optic nerve
• Species: Canine
• Lot: 7575-2

Comments

• Comments: NB300-217 was incubated at 1:10000 for 1hr at RT.

reviewed by: Verified Customer ICC Feline 05/10/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: optic nerve
• Species: Feline
• Lot: 2796-6

FAQs for NF-H Antibody (NB300-217). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of this product?
   • For NB300-217 Lot: 27967, the concentration is 22.5mg/ml (Total IgY prep).

Bioinformatics

• Gene Symbol: NEFH
• Entrez:
  • 4744(Human)
  • 380684(Mouse)
  • 24587(Rat)
• Uniprot:
  • P12036()