NF-H Antibody (CL2671) - BSA Free

Western Blot: NF-H Antibody (2671) [NBP2-46615] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue

Immunohistochemistry: NF-H Antibody (2671) [NBP2-46615] - Staining of human tonsil shows absence of immunoreactivity (negative control).

Immunohistochemistry: NF-H Antibody (2671) [NBP2-46615] - Staining of human cerebellum shows moderate immunoreactivity in Purkinje cells and neural fibers.

Immunohistochemistry: NF-H Antibody (2671) [NBP2-46615] - Staining of human cerebral cortex shows moderate positivity in neural fibers.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: CL2671
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NF-H Antibody (CL2671) - BSA Free Summary

• Description: Novus Biologicals Mouse NF-H Antibody (CL2671) - BSA Free (NBP2-46615) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P12036, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  NF-H Recombinant Protein Antigen (NBP2-46615PEP)

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for NF-H Antibody (CL2671) - BSA Free

• 200 kDa neurofilament protein
• KIAA0845
• NEFH
• Neurofilament Heavy (200kDa)
• neurofilament heavy polypeptide
• Neurofilament triplet H protein
• neurofilament, heavy polypeptide 200kDa
• neurofilament, heavy polypeptide
• NF200
• NFH
• NF-H

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEFH
• Entrez:
  • 4744(Human)
• Uniprot:
  • P12036()