Neutrophil Elastase/ELA2 Antibody (6B6G6)

Images

 
Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis of K562 cells using ELANE mouse mAb (green) and negative control (red).
Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)
Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mouse mAb against HL-60 (1), THP-1 (2), MOLT4 (3), C6 (4), and K562 (5) cell lysate.
ELISA: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow
Clone
6B6G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Neutrophil Elastase/ELA2 Antibody (6B6G6) Summary

Immunogen
Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ELANE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
Theoretical MW
28.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Neutrophil Elastase/ELA2 Antibody (6B6G6)

  • Bone marrow serine protease
  • EC 3.4.21
  • EC 3.4.21.37
  • ELA2
  • ELA2granulocyte-derived elastase
  • ELANE
  • elastase 2, neutrophil
  • elastase, neutrophil expressed
  • Elastase-2
  • GE
  • HLEelastase-2
  • HNE
  • Human leukocyte elastase
  • Leukocyte Elastase
  • Medullasin
  • NE
  • Neutrophil Elastase
  • PMN elastase
  • PMN-E
  • polymorphonuclear elastase
  • SCN1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ELANE