Neuropilin-1 Antibody (ST05-30)

Images

 
Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Western blot analysis of Neuropilin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in MCF-7 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI ...read more
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded mouse kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.
Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of Hela cells with Neuropilin-1 antibody (ST05-30) [NBP2-67539] at 1/50 dilution (red) compared with an unlabelled control (cells without ...read more
Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in HUVEC cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI ...read more
Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in SHG-44 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI ...read more
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human liver tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
ST05-30
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Neuropilin-1 Antibody (ST05-30) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal human Neuropilin-1. (SwissProt: O14786 Human ;SwissProt: P97333 Mouse ;SwissProt: Q9QWJ9 Rat)
Localization
Cell membrane, Secreted.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NRP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-67539 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Neuropilin-1 Antibody (ST05-30)

  • BDCA4
  • BDCA-4
  • CD304 antigen
  • CD304
  • DKFZp686A03134
  • DKFZp781F1414
  • neuropilin 1
  • Neuropilin1
  • Neuropilin-1
  • NP1
  • NRP
  • NRP1
  • transmembrane receptor
  • Vascular endothelial cell growth factor 165 receptor
  • VEGF165R

Background

Neuropilin-1 (also known as Npn-1, NRP1, neuropilin, or CD304/BDCA4) is a 923 amino acid (aa) type I transmembrane glycoprotein (theoretical molecular weight 130-140 kDa) located on human chromosome 10p11.22 that encodes multiple intact and soluble isoforms. The 623 aa extracellular domain (ECD) of this human cell surface receptor includes two CUB (complement-binding) domains, two F5/8 domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. The ECD of human Neuropilin-1 shares 92-95% aa sequence identity with mouse, rat, bovine, and canine Npn-1. The MAM domains of neuropilins are involved in the formation of homo- and hetero-oligomers in the absence of ligands. Neuropilin-1 expression is found in neuronal, endothelial and tumor cells as well as epithelial cells such as in the respiratory, gastrointestinal, lower urological tracts, thyroid, parathyroid and thymus gland (1,2).
Initially found to play an essential role in axon growth and guidance, NRP1 serves as a multifunctional co-receptor for class III semaphorins and heparin-binding members of the VEGF family, participating in regulation of angiogenesis, neuronal development, cell survival, migration and tumor-invasion. Neuropilin-1 has been implicated in viral infections and T-cell activation and is also described as a marker of regulatory T (Treg) cells (3). In COVID-19 infections, studies have shown NRP1 participates in viral infection via NRP1 neutralizing antibodies and binds the SARS-CoV-2 Spike protein, suggesting NRP1 may contribute to viral transport and viral internalization by endocytosis (4).

References

1. Wild JRL, Staton CA, Chapple K, Corfe BM. (2012) Neuropilins: Expression and Roles in the Epithelium. Int J Exp Pathol. 93(2):81-103. PMID: 22414290

2. Bielenberg DR, Pettaway CA, Takashima S, Klagsbrun M. (2006) Neuropilins in Neoplasms: Expression, Regulation, and Function. Exp Cell Res. 312(5):584-93. PMID: 16445911

3. Tordjman R, Lepelletier Y, Lemarchandel V, Cambot M, Gaulard P, Hermine O, Romeo P-H. (2002). A Neuronal Receptor, neuropilin-1, Is Essential for the Initiation of the Primary Immune Response. Nat Immunol. 3(5):477-82. PMID: 11953749

4. Coronavirus research updates: Test frequency matters more than test sensitivity for stopping outbreaks. 2002 June 26. Nature.com

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuropilin-1 Antibody (NBP2-67539)(2)

Reviews for Neuropilin-1 Antibody (NBP2-67539) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Neuropilin-1 Antibody (NBP2-67539). (Showing 1 - 3 of 3 FAQs).

  1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  2. What research areas can Neuropilin-1 antibodies be used in?
    • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.
  3. Does this product contain BSA/Azide?
    • Yes; the current Neuropilin-1 products we have that contain BSA and azide are NBP2-67539 and NBP2-24617. The former contains 1% BSA along with TBS (pH 7.4) and 40% glycerol as the buffer, and 0.05% Sodium Azide as a preservative. The latter contains 0.05% BSA along with 0.2 ml PBS as the buffer, and 0.05% sodium azide as a preservative.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NRP1