Neuropilin-1 Antibodies: Novus Biologicals

AF566

Neuropilin-1 Antibody [Unconjugated]

(10 Reviews)

100 μg

AF566

5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # <a class=

5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # 5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox">

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Neuropilin-1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL001) and counter-stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Neuropilin-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counter-stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

<b>Biological Strategies Validation. </b>Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED marker. DAPI staining (not shown) was used to mark nuclei (blue line). Sprouts from (a) untreated EBs, (b) treated with 2 ng ml−1 Tgf-beta for 1 h and(c) treated with 10 μM SB-421543 for 4 h. Scale bar, 13 μm. (d,e) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Fig. 10. A representative blot of six is shown; P=0.0012 NRP1 siRNA compared with control. (f,g) Proteins from P4 HUVEC transfected with control-GFP and NRP1–GFP–His construct for 24 h, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h were assessed for SMAD2 phosphorylation. Full western blots are shown in Supplementary Fig. 11. A representative blot of four is shown; P=0.0017 NRP1 overexpression compared with control. (e,g) Quantification of pSMAD2 protein normalized to SMAD2/3. (h–k) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Figs 12 and 13. (i) Quantification of pSMAD2 protein normalized to SMAD2/3; P=0.0848 Nrp1 siRNA compared with control. (k) Quantification of pSMAD3 protein normalized to SMAD3; P=0.0181 NRP1 siRNA compared with control. (l,m) Western blot analysis of P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 10 ng ml−1 BMP9 for 15 and 30 min. Full western blots are shown in Supplementary Fig. 14. A representative blot of four is shown. (m) Quantification of pSMAD2/3 protein normalized to SMAD2; P<0.0011 NRP1 siRNA compared with control. All values represent mean±s.e.m. DAPI, 4,6-diamidino-2-phenylindole; NS, not significant. Image collected

<b>Biological Strategies Validation. </b>Expression of candidate genes. (A) qPCR analysis of relative mRNA levels in total RNA from isolated glomeruli of Foxc2fl/fl; Pod‐Cre+, Foxc2fl/+; Pod‐Cre+ and Foxc2fl/+ mice. Expression levels were normalized against the expression of Rplp0. (B) qPCR analysis of relative mRNA levels in total RNA from a podocyte cell line treated with either Foxc2 siRNA or non‐targeting siRNA. (C) Western blot analysis of NRP1 and ITGB1 protein expression after Foxc2 induction (pBABE‐Foxc2) or knockdown (Foxc2 siRNA). Empty vector (pBABE) and non‐targeting siRNA was used as controls. *P < 0.05 Foxc2fl/fl;Pod‐Cre+ versus Foxc2fl/+. Error bars in (A) and (B) represent SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31062503), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED marker. DAPI staining (not shown) was used to mark nuclei (blue line). Sprouts from (a) untreated EBs, (b) treated with 2 ng ml−1 Tgf-beta for 1 h and(c) treated with 10 μM SB-421543 for 4 h. Scale bar, 13 μm. (d,e) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Fig. 10. A representative blot of six is shown; P=0.0012 NRP1 siRNA compared with control. (f,g) Proteins from P4 HUVEC transfected with control-GFP and NRP1–GFP–His construct for 24 h, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h were assessed for SMAD2 phosphorylation. Full western blots are shown in Supplementary Fig. 11. A representative blot of four is shown; P=0.0017 NRP1 overexpression compared with control. (e,g) Quantification of pSMAD2 protein normalized to SMAD2/3. (h–k) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Figs 12 and 13. (i) Quantification of pSMAD2 protein normalized to SMAD2/3; P=0.0848 Nrp1 siRNA compared with control. (k) Quantification of pSMAD3 protein normalized to SMAD3; P=0.0181 NRP1 siRNA compared with control. (l,m) Western blot analysis of P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 10 ng ml−1 BMP9 for 15 and 30 min. Full western blots are shown in Supplementary Fig. 14. A representative blot of four is shown. (m) Quantification of pSMAD2/3 protein normalized to SMAD2; P<0.0011 NRP1 siRNA compared with control. All values represent mean±s.e.m. DAPI, 4,6-diamidino-2-phenylindole; NS, not significant. Image collected

Rescue of VEGF-A Signaling in Nrp1cyto and Nrp1fl/fl Primary EC by Full-Length NRP1 and of EC Tubulogenesis following NRP1 siRNA Knockdown with Constitutively Active ERK(A and B) Western blot analysis of Nrp1cyto EC that were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (A) and quantitation of ERK activation (B) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores VEGF-A-induced VEGFR2 and ERK activation in Nrp1cyto EC (mean ± SD, n = 3, *p < 0.05).(C and D) Western blot analysis of Nrp1fl/fl EC treated with adenoviral constructs expressing CRE recombinase to inactivate NRP1 were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (C) and quantitation (D) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores the activation of VEGFR2 and ERK (mean ± SD, n = 3, *p < 0.05).(E and F) HUVEC treated with control or NRP1 siRNA were treated with the indicated adenoviral vectors to compare their capacity to undergo tube formation in 3D collagen matrices after 72 hr. Representative images (E) and quantification (F) of tubulogenesis after 72 hr (mean ± SEM, n = 18, *p < 0.05). Scale bar represents 100 um. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Impaired VEGF-A Signaling in Nrp1cyto Mice(A) Western blot analysis of heart lysates following intraperitoneal injection of VEGF-A. Blots show reduced ERK and VEGFR2 phosphorylation in Nrp1cyto mice relative to WT littermates.(B–D) Western blot analysis of cell lysates from primary arterial EC from Nrp1cyto and WT EC that were serum-starved and then stimulated for the indicated times with 50 ng/ml VEGF-A165. (B) Blots show reduced phosphorylation of VEGFR2 on Y1175 and of ERK in Nrp1cyto relative to control EC. (C) Quantification of the ratio of phospho-VEGFR2 (pVEGFR2) relative to total VEGFR2 (VEGFR2) (mean ± SD, n = 5, *p < 0.05). (D) Quantification of the ratio of phospho-ERK (pERK) relative to total ERK (ERK) (mean ± SD, n = 5, *p < 0.05).(E) Western blot analysis of cell lysates from Nrp1cyto and WT primary arterial EC that were serum-starved and stimulated for the indicated times with 50 ng/ml of FGF2 or IGF1. ERK phosphorylation was similar in Nrp1cyto and WT EC.See also Figure S4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Rescue of Defective ERK Signaling in VEGF-A-Stimulated Nrp1cyto Arterial EC by Knockdown of PTP1bPrimary arterial EC from Nrp1cyto mice transfected with siRNA specific for the indicated phosphatases were serum-starved and then stimulated with 50 ng/ml VEGF-A165.(A and B) Knockdown of the indicated phosphatases in Nrp1cyto arterial EC shown by immunoblotting (A); PTP1b knockdown was quantified in (B), dashed line indicates normal expression levels.(C and D) ERK and VEGFR2 (Y1175) phosphorylation after knockdown of the indicated phosphatases shown by immunoblotting (C). Quantification of pERK activation is shown in (D) (n = 3, mean ± SD, *p < 0.05). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF566U-100UG
Alexa Fluor 405	AF566V-100UG
Alexa Fluor 488	AF566G-100UG
Alexa Fluor 532	AF566X-100UG
Alexa Fluor 594	AF566T-100UG
Alexa Fluor 647	AF566R-100UG
Alexa Fluor 700	FAB566N
Alexa Fluor 750	AF566S-100UG
Alexa Fluor Plus 405	AF566AFP405-100UG
Alexa Fluor Plus 488	AF566AFP488-100UG
Alexa Fluor Plus 555	AF566AFP555-100UG
Alexa Fluor Plus 594	AF566AFP594-100UG
Alexa Fluor Plus 647	AF566AFP647-100UG
Alexa Fluor Plus 680	AF566AFP680-100UG
Allophycocyanin	FAB566A
Biotin	BAF566
CoraFluor 1	AF566CL1
DyLight 350	AF566D
DyLight 405	AF566E
DyLight 488	AF566K
DyLight 550	AF566L
DyLight 594	AF566M
DyLight 650	AF566W
DyLight 680	AF566Y
DyLight 755	AF566Z
Fluorescein	FAB566F
HRP	AF566H
Janelia Fluor 525	AF566JF525
Janelia Fluor 549	AF566I
Janelia Fluor 646	AF566J
Janelia Fluor 669	AF566JF669
Phycoerythrin	FAB566P
mFluor Violet 450 SE	AF566MFV450
mFluor Violet 500 SE	AF566MFV500
mFluor Violet 610 SE	AF566MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB566F
Carrier Free	FAB566P
Carrier Free	FAB566A
Carrier Free	FAB566N
Carrier Free	AF566-SP

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AF3870

Neuropilin-1 Antibody [Unconjugated]

(24 Publications)

100 μg

AF3870

HAF016). A specific band was detected for Neuropilin‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Neuropilin‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuropilin‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_lightbox">

HUVEC human umbilical vein endothelial cells were stained with Sheep Anti-Human Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3870, filled histogram) or control antibody (Catalog # <a class=

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011)." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Neuropilin-1 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and HLDM-2 human Hodgkin's lymphoma cell line (negative staining) using Sheep Anti-Human Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3870) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Sheep Anti-Human Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3870) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

<b>Genetic Strategies Validation. </b>NRP1-dependent EGFR/ERK signalling pathways are activated by EBV and are also associated with the promotion of EBV infection.(a,b) EBV infection activated EGFR/AKT and EGFR/ERK pathways. Serum-starved HNE1 cells were infected with EBV (a) or purified EBV (b) at an MOI of 5 × 103 for the indicated times. (c) EBV-activated EGFR/AKT or EGFR/ERK pathways were partially mediated by NRP1. After transfected with the indicated siRNA duplexes for 48 h, HNE1 cells were serum-starved and infected with EBV at an MOI of 5 × 103 for 1 h, followed by western blotting. For a–c, the intensity of the bands, determined with the ImageJ software, was shown as indicated. alpha -Tubulin, internal control. (d) Schematic diagram of RTKs (EGFR and VEGFR2)/NRP1/Ras/ERK and RTKs/NRP1/PI3K/AKT signalling cascade inhibitors targeting the indicated members of the pathways are shown. (e) EGFR/Ras/ERK signalling pathway was required for EBV infection. EGF-treated HNE1 and NPEC1-Bmi1 cells pre-incubated with Gefitinib (20 μM), Sorafenib (HNE1, 20 μM; NPEC1-Bmi1, 5 μM), U0126 (50 μM) and LY294002 (50 μM) for 30 min were infected with EBV for 2 h, and then cultured in the fresh medium for 48 h until FACS analysis for the infection efficiency. (f,g) Downregulation of EGFR and c-Met impaired EBV infection. HNE1 cells were transfected with siRNA duplexes targeting EGFR or c-Met for 48 h, followed by real-time PCR analysis for the expressions of EGFR and c-Met (f) or FACS analysis for EBV infection efficiency (g). (h) HRas partially rescued the inhibitory effect of Gefitinib on EBV infection. HNE1 cells transfected with pMSCV-HRAS-V12 plasmid for 24 h were pre-incubated with 20 μM Gefitinib for 30 min, followed by exposure to EBV. (i) HRas failed to rescue the suppressive effect of silencing NRP1 on EBV infection. After transfected with siNRP1 or siControl for 36 h, HNE1 cells were reseeded, and then transfected with the plasmid for HRAS-V12 or the empty vector for 24 h, followed by exposure to EBV. The infect

<b>Genetic Strategies Validation. </b>NRP1 enhances EBV infection, while NRP2 suppresses EBV infection.(a,b) Downregulation of NRP1 impaired, whereas knockdown of NRP2 promoted EBV infection. HNE1 cells were transfected with siRNA duplexes targeting NRP1 or NRP2 for 48 h, followed by NRP expression analysis by real-time PCR (a) or analysis for the efficiency of EBV infection (b); n=3. (c) EBV infection was blocked by soluble NRP1ABC, but enhanced by antibody against NRP2. For NRP1ABC protein-blocking experiment, HNE1 cells were infected with EBV, which was pre-incubated with purified NRP1ABC for 1 h. For antibody against NRP2-blocking experiment, HNE1 cells were pre-incubated with an anti-NRP2 antibody (100 μg ml−1) or goat IgG (control) at 4 °C for 1 h and then were exposed to EBV at an MOI of 5 × 103 for 3 h at 4 °C. (d,e) Overexpression of NRP1 enhanced EBV infection, while NRP2 suppressed EBV infection. HNE1 cells were transiently transfected with the expression plasmid for NRP1, NRP2 or the empty vector (pMSCV) for 24 h, followed by analysis for the expression of NRP1 and NRP2 by western blotting (d) or were exposed to EBV (e). (f,g) EGF upregulated NRP1 expression and enhanced EBV infection. HNE1 cells cultured with 10 ng ml−1 EGF for 24 h were analysed for the expression of NRP1 and NRP2 by western blotting (f) or were exposed to EBV (g). (h,i) EGF-enhanced EBV infection was partially dependent on NRP1. After transfected with siRNA against NRP1 for 48 h, EGF-treated HNE1 and NPEC-Bmi1 cells maintained in KSF medium supplemented with EGF were analysed for NRP1 expression by western blotting (h) or were exposed to EBV (i). For (b), (c), (e), (g) and (i), HNE1 or NPEC1-Bmi1 cells were exposed to EBV at an MOI of 2.5 × 103 for 2 h at 37 °C, unless otherwise indicated. The percentage of GFP-positive infected cells was analysed by FACS 48 h post infection, with controls (empty vector-transfected cells or vehicle treated-cells) set to 100%. Data represent three to five independent experiments. Values in

ST6GAL1 targeting decreases levels of a subset of N-glycoproteins that are known BTIC regulators.(A) Schematic of proteomic analysis of D456 BTICs with and without ST6GAL1 KD (n = 4 for each group of shNT, sh32, and sh33). IB with samples independent of the proteomic analysis verified that successful targeting ST6GAL1 resulted in decreased (B) PDGFRB, (C) ALCAM, and (D) NRP1 protein. (E) Schematic of pulldown using SNA-bound Agarose beads. (F) SNA pulldown and protein A/G bound agarose beads as a control demonstrated that PDGFRB, ALCAM, and NRP1 were targets for alpha 2,6 sialylation. (G) SNA pulldown of D456 PDX cells with ST6GAL1 KD compared with NT, illustrating differential pulldown of PDGFRB. (H) PDGF-BB–induced (10 minutes) activation of PDGFRB in D456 GBM PDX cells with ST6GAL1 KD compared with NT; IB for p-PDGFRB and total PDGFRB. The experiments were repeated in at least 3 independent biological replicates. Data from 1 representative experiment are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36345944), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3870U-100UG
Alexa Fluor 405	AF3870V-100UG
Alexa Fluor 488	AF3870G-100UG
Alexa Fluor 532	AF3870X-100UG
Alexa Fluor 594	AF3870T-100UG
Alexa Fluor 647	AF3870R-100UG
Alexa Fluor 700	AF3870N-100UG
Alexa Fluor 750	AF3870S-100UG
Alexa Fluor Plus 405	AF3870AFP405-100UG
Alexa Fluor Plus 488	AF3870AFP488-100UG
Alexa Fluor Plus 555	AF3870AFP555-100UG
Alexa Fluor Plus 594	AF3870AFP594-100UG
Alexa Fluor Plus 647	AF3870AFP647-100UG
Alexa Fluor Plus 680	AF3870AFP680-100UG
Allophycocyanin	AF3870A
Biotin	AF3870B
CoraFluor 1	AF3870CL1
DyLight 350	AF3870D
DyLight 405	AF3870E
DyLight 488	AF3870K
DyLight 550	AF3870L
DyLight 594	AF3870M
DyLight 650	AF3870W
DyLight 680	AF3870Y
DyLight 755	AF3870Z
FITC	AF3870F
HRP	AF3870H
Janelia Fluor 525	AF3870JF525
Janelia Fluor 549	AF3870I
Janelia Fluor 646	AF3870J
Janelia Fluor 669	AF3870JF669
PE	AF3870P
PerCP	AF3870C
mFluor Violet 450 SE	AF3870MFV450
mFluor Violet 500 SE	AF3870MFV500
mFluor Violet 610 SE	AF3870MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3870-SP

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NBP2-67539

Neuropilin-1 Antibody (ST05-30)

(2 Publications)

100 ul

NBP2-67539

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded mouse kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.

Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of Hela cells with Neuropilin-1 antibody (ST05-30) [NBP2-67539] at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in HUVEC cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in SHG-44 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human liver tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.

Western blot analysis on different lysates with NBP2-67539 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: A549 cell lysate Lane 3: SK-Br-3 cell lysate (low expression) Lane 4: MDA-MB-231 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67539 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis on different lysates with NBP2-67539 at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: SK-Br-3 cell lysate (low expression) Lane 4: Mouse brain tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: Lane 1-3: 1 minute 21 seconds; Lane 4-7: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67539 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB3870

Neuropilin-1 Antibody (446921) [Unconjugated]

(1 Review)

(5 Publications)

100 μg

MAB3870

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="HUVEC human umbilical vein endothelial cells were stained with Human Neuropilin-1 Monoclonal Antibody (Catalog # MAB3870, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3870U-100UG
Alexa Fluor 405	FAB3870V-100UG
Alexa Fluor 488	FAB3870G-100UG
Alexa Fluor 532	FAB3870X
Alexa Fluor 594	FAB3870T-100UG
Alexa Fluor 647	FAB3870R-100UG
Alexa Fluor 700	FAB3870N-100UG
Alexa Fluor 750	FAB3870S-100UG
Alexa Fluor Plus 405	FAB3870AFP405-100UG
Alexa Fluor Plus 488	FAB3870AFP488-100UG
Alexa Fluor Plus 555	FAB3870AFP555-100UG
Alexa Fluor Plus 594	FAB3870AFP594-100UG
Alexa Fluor Plus 647	FAB3870AFP647-100UG
Alexa Fluor Plus 680	FAB3870AFP680-100UG
Allophycocyanin	FAB3870A-025
Allophycocyanin	FAB3870A-100
Allophycocyanin/Cy7	FAB3870APCCY7
Biotin	FAB3870B
CoraFluor 1	FAB3870CL1
DyLight 350	FAB3870D
DyLight 405	FAB3870E
DyLight 488	FAB3870K
DyLight 550	FAB3870L
DyLight 594	FAB3870M
DyLight 650	FAB3870W
DyLight 680	FAB3870Y
DyLight 755	FAB3870Z
Fluorescein	FAB3870F-100
HRP	FAB3870H
Janelia Fluor 525	FAB3870JF525
Janelia Fluor 549	FAB3870I
Janelia Fluor 646	FAB3870J
Janelia Fluor 669	FAB3870JF669
PE/Atto594	FAB3870PEATT594
PE/Cy5.5	FAB3870PECY55
PE/Cy7	FAB3870PECY7
Phycoerythrin	FAB3870P-100
Phycoerythrin	FAB3870P-025
mFluor Violet 450 SE	FAB3870MFV450
mFluor Violet 500 SE	FAB3870MFV500
mFluor Violet 610 SE	FAB3870MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3870-SP
Carrier Free	FAB3870P-100
Carrier Free	FAB3870P-025
Carrier Free	FAB3870F-100
Carrier Free	FAB3870A-100
Carrier Free	FAB3870A-025
Carrier Free	FAB3870V-100UG
Carrier Free	FAB3870U-100UG
Carrier Free	FAB3870T-100UG
Carrier Free	FAB3870S-100UG
Carrier Free	FAB3870R-100UG
Carrier Free	FAB3870G-100UG
Carrier Free	FAB3870N-100UG

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MAB59941

Neuropilin-1 Antibody (761704)

(5 Publications)

100 μg

MAB59941

FAB554A) and either (A) Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB59941) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_lightbox">

Mouse splenocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # <a class=

FAB554A) and either (A) Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB59941) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Mouse splenocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB59941) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB59941U-100UG
Alexa Fluor 405	FAB59941V-100UG
Alexa Fluor 488	FAB59941G-100UG
Alexa Fluor 532	FAB59941X
Alexa Fluor 594	FAB59941T-100UG
Alexa Fluor 647	FAB59941R-100UG
Alexa Fluor 700	FAB59941N-100UG
Alexa Fluor 750	FAB59941S-100UG
Alexa Fluor Plus 405	FAB59941AFP405-100UG
Alexa Fluor Plus 488	FAB59941AFP488-100UG
Alexa Fluor Plus 555	FAB59941AFP555-100UG
Alexa Fluor Plus 594	FAB59941AFP594-100UG
Alexa Fluor Plus 647	FAB59941AFP647-100UG
Alexa Fluor Plus 680	FAB59941AFP680-100UG
Allophycocyanin	FAB59941A
Allophycocyanin/Cy7	FAB59941APCCY7
Biotin	FAB59941B
CoraFluor 1	FAB59941CL1
DyLight 350	FAB59941D
DyLight 405	FAB59941E
DyLight 488	FAB59941K
DyLight 550	FAB59941L
DyLight 594	FAB59941M
DyLight 650	FAB59941W
DyLight 680	FAB59941Y
DyLight 755	FAB59941Z
FITC	FAB59941F
HRP	FAB59941H
Janelia Fluor 525	FAB59941JF525
Janelia Fluor 549	FAB59941I
Janelia Fluor 646	FAB59941J
Janelia Fluor 669	FAB59941JF669
PE	FAB59941P
PE/Atto594	FAB59941PEATT594
PE/Cy5.5	FAB59941PECY55
PE/Cy7	FAB59941PECY7
PerCP	FAB59941C
mFluor Violet 450 SE	FAB59941MFV450
mFluor Violet 500 SE	FAB59941MFV500
mFluor Violet 610 SE	FAB59941MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB59941-SP
Carrier Free	FAB59941G-100UG
Carrier Free	FAB59941V-100UG
Carrier Free	FAB59941U-100UG
Carrier Free	FAB59941T-100UG
Carrier Free	FAB59941S-100UG
Carrier Free	FAB59941N-100UG
Carrier Free	FAB59941R-100UG

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MAB5661

Neuropilin-1 Antibody (130604)

(3 Publications)

500 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5661U
Alexa Fluor 405	FAB5661V-100UG
Alexa Fluor 488	FAB5661G-100UG
Alexa Fluor 532	FAB5661X
Alexa Fluor 594	FAB5661T
Alexa Fluor 647	FAB5661R-100UG
Alexa Fluor 700	FAB5661N-100UG
Alexa Fluor 750	FAB5661S-100UG
Alexa Fluor Plus 405	FAB5661AFP405-100UG
Alexa Fluor Plus 488	FAB5661AFP488-100UG
Alexa Fluor Plus 555	FAB5661AFP555-100UG
Alexa Fluor Plus 594	FAB5661AFP594-100UG
Alexa Fluor Plus 647	FAB5661AFP647-100UG
Alexa Fluor Plus 680	FAB5661AFP680-100UG
Allophycocyanin	FAB5661A
Biotin	FAB5661B
CoraFluor 1	FAB5661CL1
DyLight 350	FAB5661D
DyLight 405	FAB5661E
DyLight 488	FAB5661K
DyLight 550	FAB5661L
DyLight 594	FAB5661M
DyLight 650	FAB5661W
DyLight 680	FAB5661Y
DyLight 755	FAB5661Z
FITC	FAB5661F
HRP	FAB5661H
Janelia Fluor 525	FAB5661JF525
Janelia Fluor 549	FAB5661I
Janelia Fluor 646	FAB5661J
Janelia Fluor 669	FAB5661JF669
PE	FAB5661P
PerCP	FAB5661C
mFluor Violet 450 SE	FAB5661MFV450
mFluor Violet 500 SE	FAB5661MFV500
mFluor Violet 610 SE	FAB5661MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5661-SP
Carrier Free	FAB5661V-100UG
Carrier Free	FAB5661S-100UG
Carrier Free	FAB5661R-100UG
Carrier Free	FAB5661N-100UG
Carrier Free	FAB5661G-100UG

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MAB38701

Neuropilin-1 Antibody (446915)

(4 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB38701U-100UG
Alexa Fluor 405	FAB38701V-100UG
Alexa Fluor 488	FAB38701G-100UG
Alexa Fluor 532	FAB38701X-100UG
Alexa Fluor 594	FAB38701T-100UG
Alexa Fluor 647	FAB38701R-100UG
Alexa Fluor 700	FAB38701N-100UG
Alexa Fluor 750	FAB38701S-100UG
Alexa Fluor Plus 405	FAB38701AFP405-100UG
Alexa Fluor Plus 488	FAB38701AFP488-100UG
Alexa Fluor Plus 555	FAB38701AFP555-100UG
Alexa Fluor Plus 594	FAB38701AFP594-100UG
Alexa Fluor Plus 647	FAB38701AFP647-100UG
Alexa Fluor Plus 680	FAB38701AFP680-100UG
Allophycocyanin	FAB38701A
Biotin	FAB38701B
CoraFluor 1	FAB38701CL1
DyLight 350	FAB38701D
DyLight 405	FAB38701E
DyLight 488	FAB38701K
DyLight 550	FAB38701L
DyLight 594	FAB38701M
DyLight 650	FAB38701W
DyLight 680	FAB38701Y
DyLight 755	FAB38701Z
FITC	FAB38701F
HRP	FAB38701H
Janelia Fluor 525	FAB38701JF525
Janelia Fluor 549	FAB38701I
Janelia Fluor 646	FAB38701J
Janelia Fluor 669	FAB38701JF669
PE	FAB38701P
PerCP	FAB38701C
mFluor Violet 450 SE	FAB38701MFV450
mFluor Violet 500 SE	FAB38701MFV500
mFluor Violet 610 SE	FAB38701MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB38701-SP

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DDX0440P-100

Neuropilin-1 Antibody (211H6.01) - Azide and BSA Free

(1 Publication)

0.1 mg

DDX0440P-100

Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - FACS analysis of human PBMC using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].

Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - FACS analysis of human monocyte-derived DC using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].

Reactivity:

Hu, Ca

Species Glossary

Reactivity: Hu, Ca

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0440A488-100
Alexa Fluor 546	DDX0440A546-100
Alexa Fluor 647	DDX0440A647-100
Biotin	DDX0440B-100

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MAB566

Neuropilin-1 Antibody (130603)

(3 Publications)

500 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB566U-100UG
Alexa Fluor 405	FAB566V
Alexa Fluor 488	FAB566G
Alexa Fluor 532	FAB566X
Alexa Fluor 594	FAB566T-100UG
Alexa Fluor 647	FAB566R
Alexa Fluor 750	FAB566S
Alexa Fluor Plus 405	FAB566AFP405-100UG
Alexa Fluor Plus 488	FAB566AFP488-100UG
Alexa Fluor Plus 555	FAB566AFP555-100UG
Alexa Fluor Plus 594	FAB566AFP594-100UG
Alexa Fluor Plus 647	FAB566AFP647-100UG
Alexa Fluor Plus 680	FAB566AFP680-100UG
Allophycocyanin/Cy7	FAB566APCCY7
Biotin	FAB566B
CoraFluor 1	FAB566CL1
DyLight 350	FAB566D
DyLight 405	FAB566E
DyLight 488	FAB566K
DyLight 550	FAB566L
DyLight 594	FAB566M
DyLight 650	FAB566W
DyLight 680	FAB566Y
DyLight 755	FAB566Z
HRP	FAB566H
Janelia Fluor 525	FAB566JF525
Janelia Fluor 549	FAB566I
Janelia Fluor 646	FAB566J
Janelia Fluor 669	FAB566JF669
PE/Atto594	FAB566PEATT594
PE/Cy5.5	FAB566PECY55
PE/Cy7	FAB566PECY7
mFluor Violet 450 SE	FAB566MFV450
mFluor Violet 500 SE	FAB566MFV500
mFluor Violet 610 SE	FAB566MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB566-SP
Carrier Free	FAB566U-100UG
Carrier Free	FAB566T-100UG

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MAB5994

Neuropilin-1 Antibody (761705)

(2 Publications)

100 μg

MAB5994

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox">

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5994U-100UG
Alexa Fluor 405	FAB5994V-100UG
Alexa Fluor 488	FAB5994G-100UG
Alexa Fluor 532	FAB5994X
Alexa Fluor 594	FAB5994T-100UG
Alexa Fluor 700	FAB5994N-100UG
Alexa Fluor 750	FAB5994S-100UG
Alexa Fluor Plus 405	FAB5994AFP405-100UG
Alexa Fluor Plus 488	FAB5994AFP488-100UG
Alexa Fluor Plus 555	FAB5994AFP555-100UG
Alexa Fluor Plus 594	FAB5994AFP594-100UG
Alexa Fluor Plus 647	FAB5994AFP647-100UG
Alexa Fluor Plus 680	FAB5994AFP680-100UG
Allophycocyanin	FAB5994A
Allophycocyanin/Cy7	FAB5994APCCY7
Biotin	FAB5994B
CoraFluor 1	FAB5994CL1
DyLight 350	FAB5994D
DyLight 405	FAB5994E
DyLight 488	FAB5994K
DyLight 550	FAB5994L
DyLight 594	FAB5994M
DyLight 650	FAB5994W
DyLight 680	FAB5994Y
DyLight 755	FAB5994Z
FITC	FAB5994F
HRP	FAB5994H
Janelia Fluor 525	FAB5994JF525
Janelia Fluor 549	FAB5994I
Janelia Fluor 646	FAB5994J
Janelia Fluor 669	FAB5994JF669
PE/Atto594	FAB5994PEATT594
PE/Cy5.5	FAB5994PECY55
PE/Cy7	FAB5994PECY7
PerCP	FAB5994C
Phycoerythrin	FAB5994P
mFluor Violet 450 SE	FAB5994MFV450
mFluor Violet 500 SE	FAB5994MFV500
mFluor Violet 610 SE	FAB5994MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB5994N-100UG
Carrier Free	FAB5994A
Carrier Free	FAB5994P
Carrier Free	MAB5994-SP
Carrier Free	FAB5994V-100UG
Carrier Free	FAB5994U-100UG
Carrier Free	FAB5994T-100UG
Carrier Free	FAB5994S-100UG
Carrier Free	FAB5994G-100UG

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NBP2-24617

Neuropilin-1 Antibody

0.1 mg

NBP2-24617

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6360A

Alt Catalog# IMG-6360A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-85763

Neuropilin-1 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-85763

Staining of human skeletal muscle shows low positivity in myocytes as expected.

Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Staining of human colon shows moderate granular cytoplasmic positivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85763-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Neuropilin-1 Antibodies

Neuropilin-1 Antibody [Unconjugated]

Neuropilin-1 Antibody [Unconjugated]

Neuropilin-1 Antibody (ST05-30)

Neuropilin-1 Antibody (446921) [Unconjugated]

Neuropilin-1 Antibody (761704)

Neuropilin-1 Antibody (130604)

Neuropilin-1 Antibody (446915)

Neuropilin-1 Antibody (211H6.01) - Azide and BSA Free

Neuropilin-1 Antibody (130603)

Neuropilin-1 Antibody (761705)

Neuropilin-1 Antibody

Neuropilin-1 Antibody - BSA Free