NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2746-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88661-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98697-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26114-50ul | |||
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0011.jpg "Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00004760-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunohistochemistry-Paraffin-H00004760-M01-img0009.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Western Blot analysis of NEUROD1 expression in IMR-32 ( Cat # L008V1 ).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0001.jpg)
![ELISA: NeuroD1 Antibody (3H8) [H00004760-M01] - Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-ELISA-H00004760-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP3-13350] - E10.5 mouse embryo. NeuroD1 stained by NeuroD1 antibody (NBP3-13350) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Immunohistochemistry-Paraffin-NBP3-13350-img0001.jpg "Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP3-13350] - E10.5 mouse embryo. NeuroD1 stained by NeuroD1 antibody (NBP3-13350) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![NeuroD1 antibody detects NeuroD1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NeuroD1 stained by NeuroD1 antibody (NBP3-13350) diluted at 1:500.Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13350_rabbit-neurod1-pab-immunocytochemistry-immunofluorescence-261120251844109.jpg)
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Analysis in control (vector only transfected HEK293T lysate) and NEUROD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Western-Blot-NBP1-88661-img0006.jpg "Western Blot: NeuroD1 Antibody [NBP1-88661] - Analysis in control (vector only transfected HEK293T lysate) and NEUROD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of four key molecules by WB & IHC in SCLC samples. (A) Example of the positive control of the four key molecules by WB in SCLC cell lines. ASCL1 was strongly expressed in H209 cells. NeuroD1 was strongly expressed in H82 cells. Pou2F3 was strongly expressed in H526 cells & weakly expressed in H82 & SBC3 cells. YAP1 was strongly expressed in SBC3 cells. beta -actin served as an internal control. (B) Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. ASCL1 staining was found in tumor cell nuclei of H209 cells. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Pou2F3 staining with a diffuse cytoplasmic pattern was found in H82, H526, & SBC3 cells. The Pou2F3 staining intensity was weak in H82 & SBC3 cells & strong in H526 cells. YAP1 was stained with a membranous pattern in SBC3 cells. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202416114787.jpg)
![Western Blot: NeuroD1 Antibody (3D11) [H00004760-M02] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M02), clone 3D11.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3D11-Western-Blot-H00004760-M02-img0006.jpg "Western Blot: NeuroD1 Antibody (3D11) [H00004760-M02] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M02), clone 3D11.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: NeuroD1 Antibody (3D11) [H00004760-M02] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3D11-Immunohistochemistry-Paraffin-H00004760-M02-img0004.jpg)
![Sandwich ELISA: NeuroD1 Antibody (3D11) [H00004760-M02] - Detection limit for recombinant GST tagged NEUROD1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3D11-Sandwich-ELISA-H00004760-M02-img0005.jpg)
![Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP2-98697] - Immunochemical staining of human NeuroD1 in human hepatoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Immunohistochemistry-Paraffin-NBP2-98697-img0002.jpg "Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP2-98697] - Immunochemical staining of human NeuroD1 in human hepatoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: NeuroD1 Antibody [NBP2-98697] - Immunochemical staining of human NeuroD1 in human endometrium with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Immunohistochemistry-Paraffin-NBP2-98697-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (5D2) [NBP3-26114] - Staining of A549 with NeuroD1 Antibody (5D2) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 522-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26114_rabbit-neurod1-mab-5d2-28220241312219.jpg "Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (5D2) [NBP3-26114] - Staining of A549 with NeuroD1 Antibody (5D2) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 522-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: NeuroD1 Antibody (5D2) [NBP3-26114] - Overlay Peak curve showing SH-SY5Y cells stained with NeuroD1 Antibody (5D2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26114_rabbit-neurod1-mab-5d2-28220241418368.jpg)
![Western Blot: NeuroD1 Antibody [NBP2-19519] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG C: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Western-Blot-NBP2-19519-img0001.jpg "Western Blot: NeuroD1 Antibody [NBP2-19519] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG C: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NeuroD1 Antibody (5V4L8) [NBP3-33192] - Western blot analysis of lysates from mouse brain, using NeuroD1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33192_rabbit-neurod1-mab-5v4l8-91220241581274.jpg "Western Blot: NeuroD1 Antibody (5V4L8) [NBP3-33192] - Western blot analysis of lysates from mouse brain, using NeuroD1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: NeuroD1 Overexpression Lysate (Adult Normal) [NBL1-13598] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NeuroD1.](http://images.novusbio.com/fullsize/NeuroD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13598-img0002.jpg "Western Blot: NeuroD1 Overexpression Lysate (Adult Normal) [NBL1-13598] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NeuroD1.")
