Netrin-1 Antibody (126518) [Alexa Fluor® 750]

• Reactivity: Ch
• Applications: WB
• Clone: 126518
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

Netrin-1 Antibody (126518) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant chicken Netrin-1
  Gly26-Ala606
  Accession # Q90922
• Specificity: Detects chicken Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 1% cross-reactivity with recombinant chicken Netrin-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Netrin-1 Antibody (126518) [Alexa Fluor® 750]

• netrin 1
• Netrin1
• Netrin-1
• NTN1
• NTN1Lnetrin 1, mouse, homolog of

Background

Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma ‑chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chicken Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chicken netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta ‑chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q90922()