Netrin-1 Antibody (126518) [Alexa Fluor® 700]

• Applications: WB
• Clone: 126518
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Netrin-1 Antibody (126518) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant chicken Netrin-1
  Gly26-Ala606
  Accession # Q90922
• Specificity: Detects chicken Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 1% cross-reactivity with recombinant chicken Netrin-2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma ‑chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chicken Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chicken netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta ‑chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.