DCC Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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DCC Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse DCC
Phe32-Asn1097
Accession # P70211
Specificity
Detects mouse DCC in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCC
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 1 Review rated 4
using
AF844 in the following applications:

Publications
Read Publications using
AF844 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.


U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DCC Antibody

  • Colorectal cancer suppressor
  • CRC18
  • CRCR1
  • DCC
  • deleted in colorectal cancer protein
  • deleted in colorectal carcinoma
  • IGDCC1colorectal tumor suppressor
  • Immunoglobulin superfamily DCC subclass member 1
  • immunoglobulin superfamily, DCC subclass, member 1
  • netrin receptor DCC
  • Tumor suppressor protein DCC

Background

Deleted in colorectal cancer (DCC) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. This gene has also been found to be deleted in several different kinds of cancers. DCC encodes a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig-like domains and six fibronectin type III repeats. Two forms of the protein (the long and the short isoforms) are produced from the same gene by the use of alternative initiation sites. A third isoform that is produced by alternative splicing is expressed only in the embryo. The extracellular domain of mouse DCC shares 97% and 99% amino acid sequence identity with the human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple tissues. In the embryo, high levels of expression are detected in the brain and neural tube. DCC has been shown to be a receptor for the netrins that are important for axon guidance. DCC has also been shown to induce apoptosis in the absence of ligand binding and to block apoptosis when engaged by netrin-1. DCC has been shown to be a caspase substrate. The pro-apoptotic effects of DCC were found to be dependent on the proteolytic cleavage of the unoccupied receptor by caspase. It is likely that DCC functions as a tumor-suppressor gene by inducing apoptosis in cells that are not exposed to netrins.

  1. Fearon, E.R. et al. (1990) Science 247:49.
  2. Keino-Masu, K. et al. (1996) Cell 87:175.
  3. Mehlen, P. et al. (1998) Nature 395:801.
  4. Culotti, J.G. and D.C. Merz (1998) Current Opinion in Cell Biology 10:609.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DCC Antibody (AF844)(4)

Review for DCC Antibody (AF844) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF844:
Filter by Applications
IF
(1)
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Mouse
(1)
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reviewed by:
Anonymous
IF Mouse 01/09/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23089626
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23089626

Details

Detection NotesSee PMID 23089626

Comments

CommentsPublished: See PMID 23089626Independent review from antibodyresource.comVery good signal on mouse stem cell-derived neurons

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Secondary Antibodies

 

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Diseases for DCC Antibody (AF844)

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Recent Reviews

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1
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Anonymous
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol DCC
Uniprot