DCC Antibody [Unconjugated]

DCC was detected in immersion fixed paraffin-embedded sections of Mouse Embryo Developing Brain using Goat Anti-Mouse DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF844) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DCC Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse DCC
  Phe32-Asn1097
  Accession # P70211
• Specificity: Detects mouse DCC in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DCC
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California. U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DCC Antibody [Unconjugated]

• Colorectal cancer suppressor
• CRC18
• CRCR1
• DCC
• deleted in colorectal cancer protein
• deleted in colorectal carcinoma
• IGDCC1colorectal tumor suppressor
• Immunoglobulin superfamily DCC subclass member 1
• immunoglobulin superfamily, DCC subclass, member 1
• netrin receptor DCC
• Tumor suppressor protein DCC

Background

Deleted in colorectal cancer (DCC) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. This gene has also been found to be deleted in several different kinds of cancers. DCC encodes a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig-like domains and six fibronectin type III repeats. Two forms of the protein (the long and the short isoforms) are produced from the same gene by the use of alternative initiation sites. A third isoform that is produced by alternative splicing is expressed only in the embryo. The extracellular domain of mouse DCC shares 97% and 99% amino acid sequence identity with the human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple tissues. In the embryo, high levels of expression are detected in the brain and neural tube. DCC has been shown to be a receptor for the netrins that are important for axon guidance. DCC has also been shown to induce apoptosis in the absence of ligand binding and to block apoptosis when engaged by netrin-1. DCC has been shown to be a caspase substrate. The pro-apoptotic effects of DCC were found to be dependent on the proteolytic cleavage of the unoccupied receptor by caspase. It is likely that DCC functions as a tumor-suppressor gene by inducing apoptosis in cells that are not exposed to netrins.

1. Fearon, E.R. et al. (1990) Science 247:49.
2. Keino-Masu, K. et al. (1996) Cell 87:175.
3. Mehlen, P. et al. (1998) Nature 395:801.
4. Culotti, J.G. and D.C. Merz (1998) Current Opinion in Cell Biology 10:609.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCC Antibody (AF844)(12)

Publications using AF844

• Brianna Dailey-Krempel, Andrew L. Martin, Ha-Neul Jo, Harald J. Junge, Zhe Chen A tug of war between DCC and ROBO1 signaling during commissural axon guidance Cell Reports 2023-05-30 [PMID: 37149867]
• Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury Experimental Eye Research 2022-06-01 [PMID: 35427568]
• Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells STAR Protocols 2021-03-04 [PMID: 33659900]
• RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020-11-24;33(8):108425. 2020-11-24 [PMID: 33238113] (IHC, Rat)
• M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord Cell Rep, 2019-03-19;26(12):3298-3312.e4. 2019-03-19 [PMID: 30893602] (Immunoprecipitation, Mouse)
• N Ruiz-Reig, M Rakotobe, I Bethus, G Le Menn, HI Huditz, H Marie, T Lamonerie, F D'Autréaux Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits J. Neurosci., 2018-12-28;39(6):1005-1019. 2018-12-28 [PMID: 30593496] (IHC, Mouse)
• SG Varadaraja, JH Kong, KD Phan, TJ Kao, SC Panaitof, J Cardin, H Eltzschig, A Kania, BG Novitch, SJ Butler Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord Neuron, 2017-04-21;0(0):. 4/21/2017 [PMID: 28434801]
• Filip A. Konopacki, Hovy Ho-Wai Wong, Asha Dwivedy, Anaïs Bellon, Michael D. Blower, Christine E. Holt ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis Open Biology 2016-04-20 [PMID: 27248654]
• Kuwako K, Kakumoto K, Imai T Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron, 2010-08-12;67(3):407-21. 2010-08-12 [PMID: 20696379] (IHC-Fr, Western Blot, Mouse)
• Hoang S, Liauw J, Choi M Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J. Cereb. Blood Flow Metab., 2008-11-05;29(2):385-97. 2008-11-05 [PMID: 18985053] (IHC-Fr, Mouse)
• Merchan P, Bribian A, Sanchez-Camacho C, Lezameta M, Bovolenta P, De Castro F Sonic hedgehog promotes the migration and proliferation of optic nerve oligodendrocyte precursors. Mol. Cell. Neurosci., 2007-08-01;36(3):355-68. 2007-08-01 [PMID: 17826177] (Neutralization, Mouse)
• Tsai HH, Tessier-Lavigne M, Miller RH Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Development, 2003-05-01;130(10):2095-105. 2003-05-01 [PMID: 12668624] (ICC, Neutralization, Chicken, Rat)

Review for DCC Antibody (AF844) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 01/09/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23089626
• Species: Mouse

Bioinformatics

• Gene Symbol: DCC
• Uniprot:
  • P70211()