Neogenin Antibody [Unconjugated]

Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, Jurkat human acute T cell leukemia cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neogenin at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neogenin was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neogenin at approximately 207 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Subregion-specific expression of RGMs and Neogenin in the hippocampus.In situ hybridization (A–C, J–L) and immunohistochemistry (D–I, M–O) on coronal mouse brain sections at E16.5 (A–I) and P5 (J–O). Sections in D–I and M–O are counterstained in blue with fluorescent Nissl. (A–F) RGMa mRNA and protein are expressed in the ventricular zone (VZ), dentate gyrus (DG) and cornu ammonis (CA) region. Strong expression of RGMb mRNA and protein is detected in the pial surface lining the hippocampal fissure (HF). Neogenin transcripts and protein are widely expressed in the developing hippocampus (Hip). (G–I) Immunostaining with isotype matched controls. (J–L) In situ hybridization at P5 shows strong but differential expression patterns of RGMa, RGMb and Neogenin in the CA pyramidal cell layers (Pyr). In addition, strong expression of Neogenin is detected in the granular layer (GC) of the DG. (M–O) Immunohistochemistry reveals expression of RGMa and weak expression of RGMb in the stratum lacunosum moleculare (SLM) and fimbria (FIM). Neogenin strongly labels different hippocampal layers. CX, cortex; Hb, habenula; HC, hippocampal commissure; PO, polymorph layer; SO, stratum oriens; SR, stratum radiatum; Th, thalamus. Scale bar A–C: 400 µm, D–F: 300 µm, G–I: 300 µm, J–L: 500 µm and M–O: 400 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

RGM and Neogenin expression in the mouse olfactory system.In situ hybridization on coronal mouse brain sections at E16.5 (A–C′) and P5 (L–N′). Panels A′–C′ and L′–N′ show higher magnifications of boxed areas in A–C and L–N, respectively. Immunohistochemistry (D–F, H–J) and RGMa-AP section binding (G, K) on E16.5 coronal mouse brain sections. Sections in D–F and H–J are counterstained in blue with fluorescent Nissl. (A–C′) In situ hybridization shows differential expression patterns of RGMa, RGMb and Neogenin in the olfactory bulb and olfactory epithelium (OE). In line with this, immunohistochemistry reveals that axons of olfactory sensory neurons in the OE stain strongly for RGMb and weakly for RGMa and Neogenin. Furthermore, RGMa, RGMb and Neogenin are expressed on olfactory bulb axon projections such as the lateral olfactory tract (LOT). a, apical; ACa, anterior commissure pars anterior; AOB, accessory olfactory bulb; b, basal; CP, cortical plate; CRP, cribriform plate; EPL, external plexiform layer; GL, glomerular layer; GR, granule cell layer; IPL, internal plexiform layer; IZ, intermediate zone; LV, lateral ventricle; MCL, mitral cell layer; ONL, olfactory nerve layer; OVZ, olfactory ventricular zone; S, septum; STR, striatum; VN, vomeronasal nerve. Scale bar A–C 200 µm, A′–C′ 100 µm, D–F 300 µm, G 500 µm, H–J 400 µm, K 500 µm, L–N 400 µm and L′–N′ 200 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neogenin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Neogenin
  Ala42-Ile1033 (Asp442-Leu461 del)
  Accession # NP_032710
• Specificity: Detects human and mouse Neogenin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEO1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neogenin Antibody [Unconjugated]

• HsT17534
• IGDCC2DKFZp547B146
• Immunoglobulin superfamily DCC subclass member 2
• NEO
• NEO1
• neogenin (chicken) homolog 1
• neogenin 1
• neogenin homolog 1
• Neogenin
• NGN
• NGNDKFZp547A066

Background

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93%, and 86% aa sequence identity with rat, human, and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1,
‑3, -4, G1, and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

1. Keeling, S.L. et al. (1997) Oncogene 15:691.
2. Hong, K. et al. (1999) Cell 97:927.
3. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.
5. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neogenin Antibody (AF1079)(17)

Publications using AF1079

• Ochiai, Y;Kobayashi, H;Sunagawa, M;Baba, T;Malagola, E;Wu, F;Tanaka, T;Hata, M;Arai, J;Jiang, Z;White, RA;Zhi, X;Qian, J;Waterbury, QT;Tu, R;Zheng, B;Zeng, Y;Zheng, H;Zhang, P;Li, S;Zamechek, LB;LaBella, JS;Sugie, T;Iida, T;Enomoto, A;Eltzschig, HK;Palermo, CF;Chio, IIC;Olive, KP;Wang, TC; Netrin-1 promotes pancreatic tumorigenesis and innervation through NEO1 bioRxiv : the preprint server for biology 2025-07-26 [PMID: 40777309] (Immunohistochemistry, Neutralization, Mouse)
• G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 2022-10-22;23(21):. 2022-10-22 [PMID: 36361539] (Neutralization, Western Blot, Human)
• Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury Experimental Eye Research 2022-06-01 [PMID: 35427568]
• Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina et al. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling Cell 2021-04-15 [PMID: 33740419]
• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Western Blot, Mouse)
• T Hadi, L Boytard, M Silvestro, D Alebrahim, S Jacob, J Feinstein, K Barone, W Spiro, S Hutchison, R Simon, D Rateri, F Pinet, D Fenyo, M Adelman, KJ Moore, HK Eltzschig, A Daugherty, B Ramkhelawo Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells Nat Commun, 2018-11-27;9(1):5022. 2018-11-27 [PMID: 30479344] (Neutralization, Mouse)
• M Schlegel, A Körner, T Kaussen, U Knausberg, C Gerber, G Hansmann, HS Jónasdótti, M Giera, V Mirakaj Inhibition of neogenin fosters resolution of inflammation and tissue regeneration J. Clin. Invest., 2018-09-17;0(0):. 2018-09-17 [PMID: 30222138] (Apoptosis, Efferocytosis Assay, Neutralization, Stimulation, Transcriptional Analysis, Human, Mouse, Transgenic Mouse)
• J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 2018-04-09;0(0):. 2018-04-09 [PMID: 29632360] (IHC, Mouse)
• RGMB and neogenin control cell differentiation in the developing olfactory epithelium Development, 2016-05-01;143(9):1534-46. 5/1/2016 [PMID: 27143755]
• Frydlova J, Prikryl P, Truksa J, Falke L, Du X, Gurieva I, Vokurka M, Krijt J Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats. PLoS ONE, 2016-02-04;11(2):e0148540. 2016-02-04 [PMID: 26845567] (Western Blot, Mouse)
• Dianne M. A. van den Heuvel, Anita J. C. G. M. Hellemons, R. Jeroen Pasterkamp Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain PLoS ONE 2013-02-14 [PMID: 23457482]
• Konig K, Gatidou D, Granja T, Meier J, Rosenberger P, Mirakaj V The axonal guidance receptor neogenin promotes acute inflammation. PLoS ONE, 2012-03-06;7(3):e32145. 2012-03-06 [PMID: 22412855] (Neutralization, Mouse)
• Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates. J. Neurosci., 2012-01-11;32(2):411-6. 2012-01-11 [PMID: 22238077] (IHC-Fr, Mouse)
• Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis. Am. J. Pathol., 2009-08-21;175(3):1010-8. 2009-08-21 [PMID: 19700747] (IHC-P, Mouse)
• Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 2008-08-21;105(34):12491-6. 2008-08-21 [PMID: 18719102] (Western Blot, Human)
• Kuns-Hashimoto R, Kuninger D, Nili M, Rotwein P Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin. Am. J. Physiol., Cell Physiol., 2008-02-20;294(4):C994-C1003. 2008-02-20 [PMID: 18287331] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Osterfield M, Egelund R, Young LM, Flanagan JG Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system. Development, 2008-02-13;135(6):1189-99. 2008-02-13 [PMID: 18272596] (Western Blot, Chicken)

Bioinformatics

• Gene Symbol: NEO1
• Uniprot:
  • NP_032710()