Neogenin Antibody [Unconjugated]

Neogenin was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, Jurkat human acute T cell leukemia cell line, and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neogenin at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neogenin at approximately 207 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neogenin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Neogenin
  Ala42-Ile1033 (Asp442-Leu461 del)
  Accession # NP_032710
• Specificity: Detects human and mouse Neogenin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEO1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neogenin Antibody [Unconjugated]

• HsT17534
• IGDCC2DKFZp547B146
• Immunoglobulin superfamily DCC subclass member 2
• NEO
• NEO1
• neogenin (chicken) homolog 1
• neogenin 1
• neogenin homolog 1
• Neogenin
• NGN
• NGNDKFZp547A066

Background

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93%, and 86% aa sequence identity with rat, human, and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1,
‑3, -4, G1, and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

1. Keeling, S.L. et al. (1997) Oncogene 15:691.
2. Hong, K. et al. (1999) Cell 97:927.
3. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.
5. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neogenin Antibody (AF1079)(11)

Publications using AF1079

• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020;11(1):5171. 2020 [PMID: 33057002] (Western Blot, Mouse)
• T Hadi, L Boytard, M Silvestro, D Alebrahim, S Jacob, J Feinstein, K Barone, W Spiro, S Hutchison, R Simon, D Rateri, F Pinet, D Fenyo, M Adelman, KJ Moore, HK Eltzschig, A Daugherty, B Ramkhelawo Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells Nat Commun, 2018;9(1):5022. 2018 [PMID: 30479344] (Neutralization, Mouse)
• M Schlegel, A Körner, T Kaussen, U Knausberg, C Gerber, G Hansmann, HS Jónasdótti, M Giera, V Mirakaj Inhibition of neogenin fosters resolution of inflammation and tissue regeneration J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30222138] (Apoptosis, Human)
• J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 2018;0(0):. 2018 [PMID: 29632360] (IHC, Mouse)
• Frydlova J, Prikryl P, Truksa J, Falke L, Du X, Gurieva I, Vokurka M, Krijt J Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats. PLoS ONE, 2015;11(2):e0148540. 2015 [PMID: 26845567] (Western Blot, Mouse)
• Konig K, Gatidou D, Granja T, Meier J, Rosenberger P, Mirakaj V The axonal guidance receptor neogenin promotes acute inflammation. PLoS ONE, 2012;7(3):e32145. 2012 [PMID: 22412855] (Neutralization, Mouse)
• Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates. J. Neurosci., 2012;32(2):411-6. 2012 [PMID: 22238077] (IHC-Fr, Mouse)
• Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis. Am. J. Pathol., 2009;175(3):1010-8. 2009 [PMID: 19700747] (IHC-P, Mouse)
• Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 2008;105(34):12491-6. 2008 [PMID: 18719102] (Western Blot, Human)
• Kuns-Hashimoto R, Kuninger D, Nili M, Rotwein P Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin. Am. J. Physiol., Cell Physiol., 2008;294(4):C994-C1003. 2008 [PMID: 18287331] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Osterfield M, Egelund R, Young LM, Flanagan JG Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system. Development, 2008;135(6):1189-99. 2008 [PMID: 18272596] (Western Blot, Chicken)

Bioinformatics

• Gene Symbol: NEO1
• Uniprot:
  • NP_032710()