UNC5H3/UNC5C Antibody (200414)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
200414
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

UNC5H3/UNC5C Antibody (200414) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human UNC5H3
Ala40-Asp376
Accession # O95185
Specificity
Detects human UNC5H3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human UNC5H4 or recombinant rat UNC5H2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UNC5C
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publication using
MAB1005 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC5H3/UNC5C Antibody (200414)

  • netrin receptor UNC5C
  • Protein unc-5 homolog 3
  • Protein unc-5 homolog C
  • unc-5 homolog 3
  • unc-5 homolog C (C. elegans)
  • UNC5C
  • UNC5H3
  • UNC5H3unc5 (C.elegans homolog) c

Background

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). UNC5 family proteins are receptors for the Netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are Laminin-related proteins. Netrin family proteins can act as chemoattractants for some axons and as chemorepellents for others. Besides UNC5, Netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, Netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to Netrin is converted to repulsion by binding of UNC5 to the DCC-Netrin complex. In vivo, the mechanisms of Netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of Netrin (6). Human UNC5H3 cDNA encodes a 931 amino acid (aa) type I membrane protein with a putative 39 aa signal peptide and 337 aa extracellular domain. The extracellular domain of human UNC5H3 shares approximately 98%, 73%, and 66% aa sequence similarity with mouse UNC5H3, rat UNC5H2, and human UNC5H4, respectively.
  1. Hong, K. et al. (1999) Cell 97:927.
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
  4. Corset, V. (2000) Nature 407:747.
  5. Merz, D.C. (2001) Genetics 158:1071.
  6. Llambi, F. et al. (2001) EMBO J. 20:2715.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UNC5H3/UNC5C Antibody (MAB1005)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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(1)
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Bioinformatics

Gene Symbol UNC5C
Entrez
Uniprot