Recombinant Mouse Netrin-1 Protein


1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.

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Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Netrin-1 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3‑200 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Netrin-1 protein
Mouse Netrin-1
Accession # AAC52971
N-terminus C-terminus
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
68.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
83 kDa, reducing conditions
Read Publications using
1109-N1 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with BSA as a carrier protein.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Netrin-1 Protein

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of


Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma ‑chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).
  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.

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Publications for Netrin-1 (1109-N1)(39)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

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Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using 1109-N1 Applications Species
Schlienger, S;Yam, PT;Balekoglu, N;Ducuing, H;Michaud, JF;Makihara, S;Kramer, DK;Chen, B;Fasano, A;Berardelli, A;Hamdan, FF;Rouleau, GA;Srour, M;Charron, F; Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control Science advances 2023-05-12 [PMID: 37172092] (Bioassay, Rat) Bioassay Rat
P Ramalingam, MC Gutkin, MG Poulos, T Tillery, C Doughty, A Winiarski, AG Freire, S Rafii, D Redmond, JM Butler Restoring bone marrow niche function rejuvenates aged hematopoietic stem cells by reactivating the DNA Damage Response Nature Communications, 2023-04-10;14(1):2018. 2023-04-10 [PMID: 37037837] (Bioassay, Mouse) Bioassay Mouse
X Xia, Z Mao, W Wang, J Ma, J Tian, S Wang, K Yin Netrin-1 promotes the immunosuppressive activity of MDSCs in colorectal cancer Cancer Immunology Research, 2023-05-03;0(0):. 2023-05-03 [PMID: 36812256] (Bioassay, Mouse) Bioassay Mouse
Y Zang, K Chaudhari, GJ Bashaw Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs Cell Reports, 2022-12-06;41(10):111785. 2022-12-06 [PMID: 36476876] (Bioassay, Mouse) Bioassay Mouse
ES Chan, Y Ge, YW So, YF Bai, L Liu, YT Wang Allosteric potentiation of GABAA receptor single-channel conductance by netrin-1 during neuronal-excitation-induced inhibitory synaptic homeostasis Cell Reports, 2022-11-01;41(5):111584. 2022-11-01 [PMID: 36323250] (Bioassay, Mouse) Bioassay Mouse
H Wei, L Ailanen, M Morales, A Koivisto, A Pertovaara Spinal TRPA1 Contributes to the Mechanical Hypersensitivity Effect Induced by Netrin-1 International Journal of Molecular Sciences, 2022-06-14;23(12):. 2022-06-14 [PMID: 35743067] (Bioassay, Mouse) Bioassay Mouse
V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice Stem Cell Research, 2022-02-25;60(0):102723. 2022-02-25 [PMID: 35247845] (Bioassay, Mouse) Bioassay Mouse
K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann Endothelial Unc5B controls blood-brain barrier integrity Nature Communications, 2022-03-04;13(1):1169. 2022-03-04 [PMID: 35246514] (Bioassay, Mouse) Bioassay Mouse
D Pradella, G Deflorian, A Pezzotta, A Di Matteo, E Belloni, D Campolungo, A Paradisi, M Bugatti, W Vermi, M Campioni, A Chiapparin, L Scietti, F Forneris, C Giampietro, N Volf, M Rehman, S Zacchigna, MP Paronetto, A Pistocchi, A Eichmann, P Mehlen, C Ghigna A ligand-insensitive UNC5B splicing isoform regulates angiogenesis by promoting apoptosis Nature Communications, 2021-08-11;12(1):4872. 2021-08-11 [PMID: 34381052] (Bioassay, Human) Bioassay Human
G Chen, SS Kang, Z Wang, EH Ahn, Y Xia, X Liu, IM Sandoval, FP Manfredsso, Z Zhang, K Ye Netrin-1 receptor UNC5C cleavage by active &amp;delta-secretase enhances neurodegeneration, promoting Alzheimer&#039;s disease pathologies Science Advances, 2021-04-16;7(16):. 2021-04-16 [PMID: 33863723] (Bioassay, Rat) Bioassay Rat
Show All 39 Publications.

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Gene Symbol Ntn1