Recombinant Mouse Netrin-1 Protein

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1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Netrin-1 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3‑200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Netrin-1 protein
Mouse Netrin-1
(Val22-Ala603)
Accession # AAC52971
IEGR GGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Val22
Protein/Peptide Type
Recombinant Proteins
Gene
Ntn1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
68.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
83 kDa, reducing conditions
Publications
Read Publications using
1109-N1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Netrin-1 Protein

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma ‑chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).
  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.

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1109-N1
Species: Mu
Applications: Binding Activity

Publications for Netrin-1 (1109-N1)(23)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(16)
In Vivo
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(18)
Rat
(1)
Bovine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 1109-N1 Applications Species
Y Zhang, P Chen, G Di, X Qi, Q Zhou, H Gao Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor Sci Rep, 2018;8(1):5994. 2018 [PMID: 29662125] (Mouse) Mouse
T Sato, S Kokabu, Y Enoki, N Hayashi, M Matsumoto, M Nakahira, M Sugasawa, T Yoda Functional Roles of Netrin-1 in Osteoblast Differentiation In Vivo, 2017;31(3):321-328. 2017 [PMID: 28438858] (Bioassay, Mouse) Bioassay Mouse
K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells Neuropharmacology, 2017;119(0):26-39. 2017 [PMID: 28373074] (Bioassay, Mouse) Bioassay Mouse
KA Glendining, D Markie, RJ Gardner, EA Franz, SP Robertson, CL Jasoni A novel role for the DNA repair gene Rad51 in Netrin-1 signalling Sci Rep, 2017;7(0):39823. 2017 [PMID: 28057929] (Bioassay, Mouse) Bioassay Mouse
Y Hashimoto, Y Toyama, S Kusakari, M Nawa, M Matsuoka An Alzheimer&#039;s disease-linked rare mutation potentiates netrin receptor uncoordinated-5C-induced signaling that merges with amyloid ? precursor protein signaling J Biol Chem, 2016;0(0):. 2016 [PMID: 27068745] (Bioassay, Human) Bioassay Human
Ros O, Cotrufo T, Martinez-Marmol R, Soriano E Regulation of patterned dynamics of local exocytosis in growth cones by netrin-1. J Neurosci, 2015;35(13):5156-70. 2015 [PMID: 25834042] (Bioassay, Rat) Bioassay Rat
Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation. J Bone Miner Res, 2015;30(5):837-54. 2015 [PMID: 25483983] (Bioassay, Mouse) Bioassay Mouse
O'Leary C, Bradford D, Chen M, White A, Blackmore D, Cooper H The Netrin/RGM receptor, Neogenin, controls adult neurogenesis by promoting neuroblast migration and cell cycle exit. Stem Cells, 2015;33(2):503-14. 2015 [PMID: 25308084] (Bioassay, Mouse) Bioassay Mouse
Lu, Xi, Kim-Han, Jeong So, Harmon, Steve, Sakiyama-Elbert, Shelly E, O'Malley, Karen L The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons. Mol Neurodegener, 2014;9(0):17. 2014 [PMID: 24885281] (Bioassay, Mouse) Bioassay Mouse
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014;20(4):377-84. 2014 [PMID: 24584118] (Bioassay, Mouse) Bioassay Mouse
Show All 23 Publications.

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Bioinformatics

Gene Symbol Ntn1
Entrez
Uniprot