RGM-A Antibody [Unconjugated]

RGM-A was detected in immersion fixed paraffin-embedded sections of human brain (spinal cord) using Goat Anti-Human RGM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2459) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

RGM-A Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RGM-A
  Cys48-Gly422
  Accession # Q96B86
• Specificity: Detects human RGM-A in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RGMA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RGM-A Antibody [Unconjugated]

• RGM domain family member A
• RGM domain family, member A
• RGMA
• RGM-A
• RGMrepulsive guidance molecule A

Background

Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).

1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
2. Monnier P. et al. (2002) Nature 419:392.
3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
7. Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGM-A Antibody (AF2459)(4)

Publications using AF2459

• Fengliang Song, Zhao Yang, Liang Li, Yanping Wei, Xuewu Tang, Shuowu Liu et al. MiR-552-3p promotes malignant progression of gallbladder carcinoma by reactivating the Akt/ beta -catenin signaling pathway due to inhibition of the tumor suppressor gene RGMA Annals of Translational Medicine 9/1/2021 [PMID: 34733926]
• Bárbara S. Casas, Christelle Adolphe, Pablo Lois, Nelson Navarrete, Natalia Solís, Eva Bustamante et al. Downregulation of the Sonic Hedgehog/Gli pathway transcriptional target Neogenin-1 is associated with basal cell carcinoma aggressiveness Oncotarget 10/13/2017 [PMID: 29137400]
• Bárbara S. Casas, Christelle Adolphe, Pablo Lois, Nelson Navarrete, Natalia Solís, Eva Bustamante et al. Downregulation of the Sonic Hedgehog/Gli pathway transcriptional target Neogenin-1 is associated with basal cell carcinoma aggressiveness Oncotarget 2017-10-13 [PMID: 29137400] (Immunohistochemistry, Human, Mouse)
• AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration Oncotarget, 2017-02-07;8(6):9767-9782. 2017-02-07 [PMID: 28038459] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RGMA
• Uniprot:
  • Q96B86()