Neogenin Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Neogenin Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Neogenin
  Ala42-Ile1033 with a Asp442-Leu461 deletion
  Accession # NP_032710
• Specificity: Detects mouse Neogenin in Western blots. In this format, approximately 5% cross-reactivity with recombinant mouse DCC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEO1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order toobtain a license to use this product for such purposes, contact The Regents of the University of California. U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending. This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neogenin Antibody [Biotin]

• HsT17534
• IGDCC2DKFZp547B146
• Immunoglobulin superfamily DCC subclass member 2
• NEO
• NEO1
• neogenin (chicken) homolog 1
• neogenin 1
• neogenin homolog 1
• Neogenin
• NGN
• NGNDKFZp547A066

Background

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93% and 86% aa sequence identity with rat, human and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin-1, -3, -4, G1 and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

1. Keeling, S.L. et al. (1997) Oncogene 15:691.
2. Hong, K. et al. (1999) Cell 97:927.
3. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.
5. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEO1
• Uniprot:
  • NP_032710()