RGM‑C/Hemojuvelin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse RGM‑C/Hemojuvelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3720) at 10 ...read more
Mouse myeloma cell line NS0-derived recombinant human RGM-C isoform a (R&D Systems, Catalog # 3720-RG) Gln36-Asp400 Accession # Q6ZVN8
Detects human and mouse RGM-C in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) RGM-B is observed and less than 5% cross-reactivity with rhRGM-A is observed.
Immunogen affinity purified
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for RGM-C/Hemojuvelin Antibody
hemochromatosis type 2 (juvenile)
HJVHemochromatosis type 2 protein
repulsive guidance molecule c
RGMCRGM domain family member C
RGM-C, also known as hemojuvelin, is a member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane glycoproteins (1, 2). RGM-C is expressed in striated muscle and periportal hepatocytes (3-5). The protein undergoes partial cleavage intracellularly, resulting in a disulfide-linked dimer of the 14 kDa N-terminal and 33 kDa C-terminal portions (4, 6, 7). The N-terminal fragment contains an RGD motif, while the C-terminal fragment carries the GPI attachment site (4, 7). Two alternatively spliced isoforms lack either approximately half or the entire N-terminal fragment. Full length RGM-C can also be released from the cell and circulates in the blood (6, 8). RGM-C is disrupted in type 2A juvenile hemochromatosis, a hereditary iron homeostasis disorder characterized by excessive iron accumulation (5). In mouse, loss of RGM-C function results in decreased expression of the iron regulatory hormone hepicidin and increased iron deposition in liver, pancreas, and heart (5, 9). Membrane associated RGM-C upregulates hepicidin while soluble RGM-C downregulates hepicidin expression (8). This appears to be an iron-responsive regulatory system, as high blood iron levels reduce the amount of soluble RGM-C produced (8). RGM-C, similar to RGM-A, associates with neogenin (7). Disease-related point mutations can prevent internal RGM-C cleavage or its ability to interact with neogenin (6, 7). Experimental inflammatory conditions result in decreased RGM-C expression and increased hepicidin expression, although the two effects occur independently (5, 10). RGM-C also functions as a BMP coreceptor and enhances BMP-2 and BMP-4 signaling (11). In this context, RGM-C enhances the BMP-2 upregulation of hepatic hepicidin (11). Mature human RGM-C shares 89% amino acid (aa) sequence identity with mouse and rat RGM-C. It shares 49% and 44% aa sequence identity with human RGM-A and RGM-B, respectively.
Papanikolaou, G. et al. (2004) Nat. Genet. 36:77.
Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
Niederkofler, V. et al. (2005) J. Clin. Invest. 115:2180.
Kuninger, D. et al. (2006) J. Cell Sci. 119:3273.
Zhang, A.S. et al. (2005) J. Biol. Chem. 280:33885.
Lin, L. et al. (2005) Blood 106:2884.
Huang, F.W. et al. (2005) J. Clin. Invest. 115:2187.
Krijt, J. et al. (2004) Blood 104:4308.
Babitt, J.L. et al. (2006) Nat. Genet. 38:531.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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