NDUFB10 Antibody

Immunocytochemistry/Immunofluorescence: NDUFB10 Antibody [NBP2-19503] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: NDUFB10 Antibody [NBP2-19503] - Human breast cancer, using NDUFB10 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: NDUFB10 Antibody [NBP2-19503] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (NBP2-19503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: NDUFB10 Antibody [NBP2-19503] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (NBP2-19503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NDUFB10 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human NDUFB10. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
• Predicted Species: Primate (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFB10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Rhesus Monkey (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NDUFB10 Antibody

• CI-PDSW
• complex I PDSW subunit
• Complex I-PDSW
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10 (22kD, PDSW)
• NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
• NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
• NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3)
• NADH-ubiquinone oxidoreductase PDSW subunit
• PDSW

Background

NDUFB10 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Complex I is composed of 45 different subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NDUFB10
• Uniprot:
  • O96000(Human)