MYL7 Antibody (OTI4E7)

Western Blot: MYL7 Antibody (4E7) [NBP2-03891] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL7.

Immunohistochemistry-Paraffin: MYL7 Antibody (4E7) [NBP2-03891] Staining of paraffin-embedded Human liver tissue using anti-MYL7 mouse monoclonal antibody.

Flow Cytometry: MYL7 Antibody (4E7) [NBP2-03891] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYL7 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: MYL7 Antibody (4E7) [NBP2-03891] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MYL7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MYL7 Antibody (4E7) [NBP2-03891] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MYL7 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MYL7 Antibody (4E7) [NBP2-03891] - Staining of paraffin-embedded Human Kidney tissue using anti-MYL7 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI4E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MYL7 Antibody (OTI4E7) Summary

• Description: Novus Biologicals Mouse MYL7 Antibody (OTI4E7) (NBP2-03891) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-MYL7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human MYL7 (NP_067046) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYL7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 19.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MYL7 Antibody (OTI4E7)

• atrial isoform
• light polypeptide 7, regulatory
• MLC-2a
• MYL2A
• MYLC2AMLC2a
• myosin, light chain 7, regulatory

Background

Myosins are a large family of motor proteins that share the common features of ATP hydrolysis, actin binding andpotential for kinetic energy transduction. Originally isolated from muscle cells (hence the name), almost alleukaryotic cells are now known to contain myosins. Structurally, mysoins contain a head domain that binds to actinfilaments (microfilaments) and is the site of ATP hydrolysis. The tail domain interacts with cargo molecules, and theneck acts as a linker between the head and tail and is the site of regulatory myosin light chain binding. There are 17myosin families and the most well characterized is myosin II. Myosin II is found predominantly in myocytes andmediates plus-ended movement along microfilaments. It is involved in muscle contraction through cyclic interactionswith actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin lightchain kinase (MLCK) and by intracellular Ca2+ concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYL7 Antibody (NBP2-03891)(1)

Publication using NBP2-03891

• Jiang L, Wang M, Lin S et al. A Quantitative Proteome Map of the Human Body Cell 2020-10-01 [PMID: 32916130] (WB, Human)
  
  Details:
  GTEx Project

Bioinformatics

• Gene Symbol: MYL7
• Entrez:
  • 58498(Human)