FHL1 Antibody (767418)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
767418
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

FHL1 Antibody (767418) Summary

Immunogen
E. coli-derived recombinant human FHL1
Thr93-Asn166
Accession # Q13642
Specificity
Detects human FHL1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FHL1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Control
FHL1 Knockout HeLa Cell Lysate
Publications
Read Publication using
MAB5938 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FHL1 Antibody (767418)

  • bA535K18.1
  • FHL1
  • four and a half LIM domains 1
  • HLH1
  • HPLH1
  • KYOT
  • KYO-T
  • LIM protein SLIMMER
  • skeletal muscle LIM-protein1
  • SLIM1
  • SLIM1Isl-1 and Mec-3 domains 1
  • SLIMMER
  • XMPMA

Background

FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is an approximately 35 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FHL1 Antibody (MAB5938)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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ICC
(1)
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(1)
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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol FHL1
Uniprot