IDH3A Antibody - Azide Free

Immunocytochemistry/Immunofluorescence: IDH3A Antibody [NBP1-32396] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IDH3A stained by IDH3A antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: IDH3A Antibody [NBP1-32396] - Human lung SCC, using IDH3A antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: IDH3A Antibody [NBP1-32396] - Analysis of paraffin-embedded zebrafish tissue, using IDH3A antibody at 1:300 dilution.

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP1-32396) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

IDH3A Antibody - Azide Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IDH3A. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Rhesus Macaque (99%), Bovine (97%), Chicken (94%), Xenopus (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IDH3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for IDH3A Antibody - Azide Free

• EC 1.1.1
• EC 1.1.1.41
• H-IDH alpha
• isocitrate dehydrogenase (NAD+) alpha chain
• isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• isocitrate dehydrogenase 3 (NAD+) alpha
• Isocitric dehydrogenase subunit alpha
• NAD(+)-specific ICDH subunit alpha
• NAD(H)-specific isocitrate dehydrogenase alpha subunit
• NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IDH3A Antibody (NBP1-32396)(2)

Publications using NBP1-32396

• Tai Y, Engels D, Locatelli G et al. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions bioRxiv 2023-04-04 (IHC, Mouse)
  
  Details:
  Dilutions: 1:400
• Tai YH, Engels D, Locatelli G et al. Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions Nature Metabolism 2023-07-10 [PMID: 37430025] (In vivo assay)

Bioinformatics

• Gene Symbol: IDH3A