NCOA3/AIB1 Antibody

Images

 
Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Samples: Nuclear extract (5 ug for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 ug/ml for WB and 20 ug/10 mg extract for CoIP. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
   

Biological Strategies

     

Order Details

NCOA3/AIB1 Antibody Summary

Immunogen
A synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 16 (LocusLink ID 8202).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
Application Notes
This protein is a cytoplasmic coactivator that shifts to the nucleus upon activation. It can be analyzed on Western blot using nuclear or cytosolic extracts.
Positive Control
HeLa Lysate (NB800-PC9)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NCOA3/AIB1 Antibody

  • ACTR
  • ACTRCBP-interacting protein
  • AIB1
  • AIB1AIB-1
  • Amplified in breast cancer 1 protein
  • BHLHE42
  • bHLHe42p/CIP
  • CAGH16
  • Class E basic helix-loop-helix protein 42
  • CTG26
  • KAT13B
  • MGC141848
  • NCOA3
  • NCoA-3
  • nuclear receptor coactivator 3
  • pCIP
  • pCIP
  • RAC3
  • RAC-3
  • RAC3pCIP
  • Receptor-associated coactivator 3
  • SRC-1
  • SRC3
  • SRC-3
  • SRC-3EC 2.3.1.48
  • Steroid receptor coactivator protein 3
  • Thyroid hormone receptor activator molecule 1
  • TNRC16
  • TRAM1
  • TRAM-1TNRC14

Background

Steroid receptor coactivator 3 (SRC3) is a ~154 kDa nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in hormone-dependent fashion. SRC3 recruits 2 other nuclear factors, CBP and PCAF, and thus plays a central role in creating a multisubunit coactivator complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NCOA3
Entrez