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![Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli and cells in tubules.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90256-img0006.jpg)
![Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256] - Staining of human lymph node shows strong nuclear positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90256-img0007.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line MCF-7](http://images.novusbio.com/fullsize/nbp1-90256_rabbit-polyclonal-ncoa3-aib1-antibody-295202516195522.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NB100-314-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NB100-314-img0002.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NBP1-77785] - Detection of AIB1 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to AIB1. Mouse AIB1(lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NBP1-77785-img0001.jpg "Western Blot: NCOA3/AIB1 Antibody [NBP1-77785] - Detection of AIB1 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to AIB1. Mouse AIB1(lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.")
![Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunohistochemistry-NBP1-77785-img0003.jpg)
![Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunohistochemistry-NBP1-77785-img0002.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NBP1-33720] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NCOA3 antibody [N1N2], N-term.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NBP1-33720-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NCOA3/AIB1 Antibody [NBP1-33720] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NCOA3 antibody [N1N2], N-term.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NCOA3/AIB1 Antibody [NBP1-33720] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33720-img0006.jpg)
![Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-33720] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunohistochemistry-Paraffin-NBP1-33720-img0007.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NBP1-33720] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NBP1-33720-img0008.jpg)
![Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 (SRC3) by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NB100-315-img0004.jpg "Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 (SRC3) by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Western-Blot-NB100-315-img0003.jpg)
![Immunoprecipitation: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 (lot 2) used for IP at 6 ug per reaction. NCOA3/AIB1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated NCOA3/AIB1, NB100-315 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NCOA3-AIB1-Antibody-Immunoprecipitation-NB100-315-img0005.jpg)
![Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody (D3) [NBP3-41658] - DAB staining on IHC-P; Sample: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41658; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41658_mouse-ncoa3-aib1-mab-d3-612202421243235.jpg "Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody (D3) [NBP3-41658] - DAB staining on IHC-P; Sample: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41658; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: AIB1 Overexpression Lysate (Adult Normal) [NBL1-13517] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AIB1.](http://images.novusbio.com/fullsize/AIB1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13517-img0002.jpg "Western Blot: AIB1 Overexpression Lysate (Adult Normal) [NBL1-13517] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AIB1.")
![ELISA: Human NCOA3/AIB1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39203] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39203_human-ncoa3-aib1-ready-to-use-elisa-kit-colorimetric-2110202415362342.png "ELISA: Human NCOA3/AIB1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39203] - Standard Curve Reference")
![ELISA: Human NCOA3/AIB1 ELISA Kit (Colorimetric) [NBP3-39202] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39202_human-ncoa3-aib1-elisa-kit-colorimetric-2110202415302991.png "ELISA: Human NCOA3/AIB1 ELISA Kit (Colorimetric) [NBP3-39202] - Standard Curve Reference")