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Immunogen | A synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 13 (LocusLink ID 8202). |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | NCOA3 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | NB 100-314 may be used for Western blot and immunoprecipitation. This protein is a cytoplasmic coactivator that shifts to the nucleus upon activation. It can be immunoprecipitated and analyzed on Western blot using nuclear or cytosolic extracts. Suggested working dilutions: * Western Blot - 1:1,000 ~ 1:10,000 (5 ug nuclear extract) Immunocytochemistry - Not Done Immunoprecipitation - 1-4 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application. |
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Storage | Store at 4C. Do not freeze. |
Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
Preservative | 0.09% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||||||||
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reviewed by:
Verified Customer |
WB | Human | 10/03/2014 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
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Secondary Antibodies |
Isotype Controls |
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