NCOA3/AIB1 Antibody

Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NCOA3/AIB1 Antibody Summary

• Immunogen: A synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 13 (LocusLink ID 8202).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 1-4 ug/mg lysate
• Application Notes: NB 100-314 may be used for Western blot and immunoprecipitation. This protein is a cytoplasmic coactivator that shifts to the nucleus upon activation. It can be immunoprecipitated and analyzed on Western blot using nuclear or cytosolic extracts. Suggested working dilutions: * Western Blot - 1:1,000 ~ 1:10,000 (5 ug nuclear extract) Immunocytochemistry - Not Done Immunoprecipitation - 1-4 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-224, NB500-160, NB200-347, NBP1-06527, NBP1-19102

Alternate Names for NCOA3/AIB1 Antibody

• ACTR
• ACTRCBP-interacting protein
• AIB1
• AIB1AIB-1
• Amplified in breast cancer 1 protein
• BHLHE42
• bHLHe42p/CIP
• CAGH16
• Class E basic helix-loop-helix protein 42
• CTG26
• KAT13B
• MGC141848
• NCOA3
• NCoA-3
• nuclear receptor coactivator 3
• pCIP
• pCIP
• RAC3
• RAC-3
• RAC3pCIP
• Receptor-associated coactivator 3
• SRC-1
• SRC3
• SRC-3
• SRC-3EC 2.3.1.48
• Steroid receptor coactivator protein 3
• Thyroid hormone receptor activator molecule 1
• TNRC16
• TRAM1
• TRAM-1TNRC14

Background

Steroid receptor coactivator 3 (SRC3) is a ~154 kDa nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in hormone-dependent fashion. SRC3 recruits 2 other nuclear factors, CBP and PCAF, and thus plays a central role in creating a multisubunit coactivator complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCOA3/AIB1 Antibody (NB100-314)(4)

Publications using NB100-314

• Lanz RB, Bulynko Y, Malovannaya A et al. Global characterization of transcriptional impact of the SRC-3 coregulator. Mol Endocrinol 2010 Apr [PMID: 20181721]
• Chen R, Liu M, Li H et al. PP2B and PP1alpha cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling. Genes Dev 2008 May [PMID: 18483222]
• Naeem H, Cheng D, Zhao Q et al. The activity and stability of the transcriptional coactivator p/CIP/SRC-3 are regulated by CARM1-dependent methylation. Mol Cell Biol 2007 Jan [PMID: 17043108]
• Li X, Lonard DM, Jung SY et al. The SRC-3/AIB1 coactivator is degraded in a ubiquitin- and ATP-independent manner by the REGgamma proteasome. Cell 2006 Jan [PMID: 16439211]

Review for NCOA3/AIB1 Antibody (NB100-314) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 10/03/2014

Summary

• Application: Western Blot
• Sample Tested: Jurkat cells
• Species: Human
• Comments: The band on interest is very faint and multiple other bands are visible but its similar to others.

Blocking

• Blocking Details: 5% milk in TBST buffer

Primary Anitbody

• Dilution Ratio: 1:500, diluted in TBST buffer+5% milk

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG-HRP (SCBT)
• Secondary Manufacturer Cat#: # sc-2004
• Secondary Concentration: 1:20,000

Details

• Detection Notes: Detected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Comments

• Comments: The band on interest is very faint and multiple other bands are visible but its similar to others.

Bioinformatics

• Gene Symbol: NCOA3
• Entrez:
  • 8202(Human)