NCOA2 Antibody

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Western Blot: NCOA2 Antibody - hole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time ...read more
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - FFPE section of prostate adenocarcinoma. Antibody used at a dilution of 1:100. Detection: DAB
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-NCOA2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - FFPE section of human breast adenocarcinoma. Antibody used at a dilution of 1:250. Detection: DAB
Immunoprecipitation: NCOA2 Antibody - Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified rabbit NCOA2 used at 20 ug/10 mg lysate. Detection: Coomassie ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NCOA2 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1400 the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-4 ug/mg
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Breast Carcinoma, Prostate Carcinoma, Testicular Seminoma. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NCOA2 Antibody

  • bHLHe75
  • bHLHe75MGC138808
  • Class E basic helix-loop-helix protein 75
  • EC 2.3.1.48
  • GRIP1glucocorticoid receptor-interacting protein-1
  • HTIF2
  • KAT13C
  • NCOA2
  • NCoA-2SRC2
  • nuclear receptor coactivator 2
  • TIF2
  • TIF2hTIF2
  • Transcriptional intermediary factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NCOA2
Entrez
Uniprot