NCOA2 Antibody - BSA Free

Western Blot: NCOA2 Antibody [NBP2-22162] - Western Blot: NCOA2 Antibody - hole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiNCOA2/ SRC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Immunoprecipitation: NCOA2 Antibody [NBP2-22162] - Immunoprecipitation: NCOA2 Antibody - Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified rabbit NCOA2 used at 20 ug/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NCOA2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NCOA2 Antibody - BSA Free (NBP2-22162) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 1400 the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-4ug/mg of Lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCOA2 Antibody - BSA Free

• bHLHe75
• bHLHe75MGC138808
• Class E basic helix-loop-helix protein 75
• EC 2.3.1.48
• GRIP1glucocorticoid receptor-interacting protein-1
• HTIF2
• KAT13C
• NCOA2
• NCoA-2SRC2
• nuclear receptor coactivator 2
• TIF2
• TIF2hTIF2
• Transcriptional intermediary factor 2

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOA2
• Entrez:
  • 64708(Human)
• Uniprot:
  • Q9H9Q2(Human)