Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.
Immunocytochemistry/ Immunofluorescence: NBR1 Antibody [NBP1-71703] - NBR1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.
Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.
Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
A genomic peptide made to an internal region of the human NBR1 protein (within residues 50-250). [Swiss-Prot Q14596]
Cytoplasm. Cytoplasmic vesicle - autophagosome. Lysosome. Cytoplasm - myofibril - sarcomere - M line. Note: In cardiac muscles localizes to the sarcomeric M line. Is targeted to lysosomes for degradation.
Immunogen affinity purified
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NBR1 (Next to BRCA1 gene 1) is a protein which is ubiquitously expressed and highly conserved in eukaryotic organisms. NBR1 is implicated in TITIN kinase signaling in muscles and mitigates FGFR signaling through NBR1- SPRED2 interactions. Animals expressing truncated NBR1 lacking the C-terminal region has been shown to develop excessive bone mass due to increased activity of osteoblasts, suggesting NBR1's role in modulating bone metabolism in vivo. NBR1 functions as a negative regulator of p38 MAPK activity and has also been discovered as a binding partner of ATG8 family members including LC3, GABARAP, GABARAP-like 1/GABARAPL1, and GATE-16/GABARAPL2 in mammalian cells wherein these proteins are known to play essential role in formation of autophagosomes. Moreover, NBR1 interacts with ubiquitin/ubiquitinated proteins via ubiquitin-associated (UBA) domain and also interacts with TRIM55, SQSTM1, RNF29, USP8 etc. Based on these properties, it has been proposed that NBR1 functions as a cargo adapter for autophagic degradation of ubiquitinated substrates in a similar way to p62.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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