NBR1 Antibody

Images

 
Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.
Immunocytochemistry/ Immunofluorescence: NBR1 Antibody [NBP1-71703] - NBR1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.
Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.
Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.24 mg/ml

NBR1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human NBR1 protein (within residues 50-250). [Swiss-Prot Q14596]
Localization
Cytoplasm. Cytoplasmic vesicle - autophagosome. Lysosome. Cytoplasm - myofibril - sarcomere - M line. Note: In cardiac muscles localizes to the sarcomeric M line. Is targeted to lysosomes for degradation.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NBR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000
  • Simple Western 1:400
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This NBR1 antibody is useful for Western blot, ICC/IF and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-71703 in the following applications:

Publications
Read Publications using
NBP1-71703 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human and mouse. Immunogen has 75% identity to the rat protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.24 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for NBR1 Antibody

  • Cell migration-inducing gene 19 protein
  • FLJ98272
  • KIAA0049CA125
  • M17S2FLJ55359,1A1-3B
  • Membrane component chromosome 17 surface marker 2,1A13B
  • membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125)
  • migration-inducing protein 19
  • Neighbor of BRCA1 gene 1 protein
  • neighbor of BRCA1 gene 1
  • next to BRCA1 gene 1 protein
  • Protein 1A1-3B

Background

NBR1 (Next to BRCA1 gene 1) is a protein which is ubiquitously expressed and highly conserved in eukaryotic organisms. NBR1 is implicated in TITIN kinase signaling in muscles and mitigates FGFR signaling through NBR1- SPRED2 interactions. Animals expressing truncated NBR1 lacking the C-terminal region has been shown to develop excessive bone mass due to increased activity of osteoblasts, suggesting NBR1's role in modulating bone metabolism in vivo. NBR1 functions as a negative regulator of p38 MAPK activity and has also been discovered as a binding partner of ATG8 family members including LC3, GABARAP, GABARAP-like 1/GABARAPL1, and GATE-16/GABARAPL2 in mammalian cells wherein these proteins are known to play essential role in formation of autophagosomes. Moreover, NBR1 interacts with ubiquitin/ubiquitinated proteins via ubiquitin-associated (UBA) domain and also interacts with TRIM55, SQSTM1, RNF29, USP8 etc. Based on these properties, it has been proposed that NBR1 functions as a cargo adapter for autophagic degradation of ubiquitinated substrates in a similar way to p62.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NBR1 Antibody (NBP1-71703)(4)

Review for NBR1 Antibody (NBP1-71703) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71703:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NBR1 NBP1-71703
Enlarge
reviewed by:
Liu Yue
WB Human and Mouse 07/14/2017
View

Summary

ApplicationWestern Blot
Sample Tested hela cell, Mouse brain
SpeciesHuman and Mouse
LotA-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NBR1 Antibody (NBP1-71703) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71703AF405
Alexa Fluor 488 Labeled NBP1-71703AF488
Alexa Fluor 647 Labeled NBP1-71703AF647
Alexa Fluor 700 Labeled NBP1-71703AF700
Biotin Labeled NBP1-71703B
DyLight 405 Labeled NBP1-71703V
DyLight 488 Labeled NBP1-71703G
DyLight 550 Labeled NBP1-71703R
DyLight 650 Labeled NBP1-71703C
FITC Labeled NBP1-71703F

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Blogs on NBR1.

Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers
The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding pr...  Read full blog post.

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Recent Reviews

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1
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Liu Yue
07/14/2017
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol NBR1
Entrez
Uniprot