Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: NBR1 Antibody [NBP1-71703] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.
Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.
Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.
Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
A genomic peptide made to an internal region of the human NBR1 protein (within residues 50-250). [Swiss-Prot Q14596]
Cytoplasm. Cytoplasmic vesicle - autophagosome. Lysosome. Cytoplasm - myofibril - sarcomere - M line. Note: In cardiac muscles localizes to the sarcomeric M line. Is targeted to lysosomes for degradation.
Immunogen affinity purified
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This NBR1 antibody is useful for Western blot, ICC/IF and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This NBR1 antibody is validated for ELISA (as capture antibody) from a verified customer review.
Read 2 Reviews rated 3.5 using NBP1-71703 in the following applications:
NBR1 (Next to BRCA1 gene 1) is a protein which is ubiquitously expressed and highly conserved in eukaryotic organisms. NBR1 is implicated in TITIN kinase signaling in muscles and mitigates FGFR signaling through NBR1- SPRED2 interactions. Animals expressing truncated NBR1 lacking the C-terminal region has been shown to develop excessive bone mass due to increased activity of osteoblasts, suggesting NBR1's role in modulating bone metabolism in vivo. NBR1 functions as a negative regulator of p38 MAPK activity and has also been discovered as a binding partner of ATG8 family members including LC3, GABARAP, GABARAP-like 1/GABARAPL1, and GATE-16/GABARAPL2 in mammalian cells wherein these proteins are known to play essential role in formation of autophagosomes. Moreover, NBR1 interacts with ubiquitin/ubiquitinated proteins via ubiquitin-associated (UBA) domain and also interacts with TRIM55, SQSTM1, RNF29, USP8 etc. Based on these properties, it has been proposed that NBR1 functions as a cargo adapter for autophagic degradation of ubiquitinated substrates in a similar way to p62.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Monitoring Autophagy in Neurons By Christina Towers, PhD. Autophagy is a critical cellular process used by most cells in the body to recycle nutrients and prevent harmful buildup of damaged proteins. It is particularly important in the brain, where ... Read full blog post.
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