NBR1 Antibody [CoraFluor™ 1]

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

NBR1 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: A genomic peptide made to an internal region of the human NBR1 protein (within residues 50-250). [Swiss-Prot Q14596]
• Localization: Cytoplasm. Cytoplasmic vesicle - autophagosome. Lysosome. Cytoplasm - myofibril - sarcomere - M line. Note: In cardiac muscles localizes to the sarcomeric M line. Is targeted to lysosomes for degradation.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NBR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA Capture
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen has 75% identity to the rat protein.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NBR1 Antibody [CoraFluor™ 1]

• Cell migration-inducing gene 19 protein
• FLJ98272
• KIAA0049CA125
• M17S2FLJ55359,1A1-3B
• Membrane component chromosome 17 surface marker 2,1A13B
• membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125)
• migration-inducing protein 19
• Neighbor of BRCA1 gene 1 protein
• neighbor of BRCA1 gene 1
• next to BRCA1 gene 1 protein
• Protein 1A1-3B

Background

NBR1 is encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled coil motif, which is present in many genes with transformation potential, but the function of this protein is unknown. This gene is located on a region of chromosome 17q21.1 that is in close proximity to tumor suppressor gene BRCA1. Three alternatively spliced variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NBR1