BAF180/PB1 Antibody

Images

 
Western Blot: BAF180/PB1 Antibody [NB100-79833] - Detection of Human and Mouse PB1/BAF180 by Western Blot. Samples : Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50ug), and Mouse NIH3T3 (50ug) cells prepared ...read more
Immunohistochemistry: BAF180/PB1 Antibody [NB100-79833] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PB1/BAF180 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. ...read more
Western Blot: BAF180/PB1 Antibody [NB100-79833] - Detection of Human and Mouse PB1/BAF180 on HeLa whole cell lysate using NB100-79833. PB1/BAF180 was immunoprecipitated by rabbit anti-PB1/BAF180 antibody NB100-79832.
Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate of HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - Section of human prostate carcinoma.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

BAF180/PB1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1639 and 1689 of human polybromo 1 (BRG1-associated factor 180) using the numbering given in entry AAP34197.1 (GeneID 55193).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PBRM1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 4
using
NB100-79833 in the following applications:

Publications
Read Publications using
NB100-79833 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAF180/PB1 Antibody

  • BAF180PB1BRG1-associated factor 180
  • hPB1
  • MGC156155
  • MGC156156
  • polybromo 1
  • polybromo-1D
  • protein polybromo-1

Background

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PB1/BAF180 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PB1/BAF180 has been proposed to function as a tumor suppressor protein. Recently, PB1/BAF180 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21. Alternate names for PB1/BAF180 include protein polybromo-1, polybromo-1D, BRG1-associated factor 180, hPB1, and PBRM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAF180/PB1 Antibody (NB100-79833)(3)

Review for BAF180/PB1 Antibody (NB100-79833) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-79833:
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Images Ratings Applications Species Date Details
Western Blot BAF180/PB1 NB100-79833
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reviewed by:
Pramod Mallikarjuna
WB Human 08/30/2014
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Summary

ApplicationWestern Blot
Sample TestedClear cell renal cell carcinoma samples
SpeciesHuman

Blocking

Blocking DetailsOdyssey Blocking Buffer

Primary Anitbody

Dilution Ratio1:1000 in TBST, overnight, +4 degree celsius

Secondary Antibody

Secondary DescriptionIRDye® 800CW Goat anti-Rabbit
Secondary Manufacturer Cat#926-32211
Secondary Concentration1:10000

Details

Detection NotesOdyssey® CLx Infrared Imaging System

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FAQs for BAF180/PB1 Antibody (NB100-79833) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for BAF180/PB1 Antibody (NB100-79833)

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Recent Reviews

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1
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Pramod Mallikarjuna
08/30/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PBRM1
Entrez
Uniprot