BAF180/PB1 Antibody

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Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. ...read more
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at ...read more
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
Independent Antibodies: Immunoprecipitation: BAF180/PB1 Antibody [NB100-79833] - Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

BAF180/PB1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1639 and 1689 of human polybromo 1 (BRG1-associated factor 180) using the numbering given in entry AAP34197.1 (GeneID 55193).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PBRM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in ICC/IF reported in scientific literature (PMID: 28092369).
Reviewed Applications
Read 1 Review rated 4
using
NB100-79833 in the following applications:

Publications
Read Publications using
NB100-79833 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAF180/PB1 Antibody

  • BAF180
  • BAF180/PB1
  • BAF180PB1BRG1-associated factor 180
  • hPB1
  • MGC156155
  • MGC156156
  • PB1
  • PBRM1
  • polybromo 1
  • Polybromo-1D
  • protein polybromo-1

Background

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PB1/BAF180 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PB1/BAF180 has been proposed to function as a tumor suppressor protein. Recently, PB1/BAF180 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21. Alternate names for PB1/BAF180 include protein polybromo-1, polybromo-1D, BRG1-associated factor 180, hPB1, and PBRM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAF180/PB1 Antibody (NB100-79833)(3)

Review for BAF180/PB1 Antibody (NB100-79833) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-79833:
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Images Ratings Applications Species Date Details
Western Blot BAF180/PB1 NB100-79833
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4
reviewed by:
Verified Customer
Western Blot Human 08/30/2014
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Summary

ApplicationWestern Blot
Sample TestedClear cell renal cell carcinoma samples
SpeciesHuman

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Secondary Antibodies

 

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Recent Reviews

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Verified Customer
08/30/2014
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol PBRM1
Entrez
Uniprot