Biological Strategies: Western Blot: BAF180/PB1 Antibody [NB100-79833] - Detection of Human and Mouse PB1/BAF180 by Western Blot. Samples : Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50ug), and ...read more
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - Mouse teratoma. Antibody: Affinity purified rabbit anti- PBRM1used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: BAF180/PB1 Antibody [NB100-79833] - Detection of Human and Mouse PB1/BAF180 on HeLa whole cell lysate using NB100-79833. PB1/BAF180 was immunoprecipitated by rabbit anti-PB1/BAF180 antibody NB100-79832.
Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PBRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: BAF180/PB1 Antibody [NB100-79833] - Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP ...read more
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PB1/BAF180 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PB1/BAF180 has been proposed to function as a tumor suppressor protein. Recently, PB1/BAF180 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21. Alternate names for PB1/BAF180 include protein polybromo-1, polybromo-1D, BRG1-associated factor 180, hPB1, and PBRM1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.