Recombinant Human CA125/MUC16 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human CA125/MUC16 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human CA125/MUC16 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin‑3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is found to be approximately 0.35-1.4 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human CA125/MUC16 protein
Met13360-Gln14347 (Met13472Thr & Gln13957Lys) with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
153-169 kDa, reducing conditions
Read Publications using
5609-MU in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CA125/MUC16 Protein, CF

  • CA125 ovarian cancer antigen
  • CA125
  • CA-125
  • CA125MUC-16
  • FLJ14303
  • MUC16
  • mucin 16, cell surface associated
  • mucin-16
  • Ovarian cancer-related tumor marker CA125
  • Ovarian carcinoma antigen CA125


MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy (1, 2). Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human MUC16 that can vary from 1148 to 22152 amino acids (aa) in length and 200 - 5000 kDa in size (1, 2). The 22152 aa form contains ser/thr-rich N-terminal tandem repeats, 4 LRR (Leu-rich repeat) domains, 56 SEA (sea urchin sperm protein, enterokinase and agrin) domains, a transmembrane domain, and a 31 aa cytoplasmic domain that includes a tyrosine phosphorylation site (1-4). SEA domains are ~120 aa in length, contain conserved residues including potential O-glycosylation sites and a pair of cysteines, and are often found in transmembrane mucins (3). The protein produced by R&D Systems represents aa 13360-14347 of the full sequence and includes the last 6 SEA domains. It shares 68% aa identity with canine MUC16. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers (5). The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity (5-7). MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells (8). MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion (9, 10). MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy (10). In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome (11).

  1. Yin, B. W. T. K. O. Lloyd, 2001, J. Biol. Chem. 276:27371.
  2. Maeda, T. et al. (2004) J. Biol. Chem. 279:13174.
  3. Fendrick, J. L. et al. (1997) Tumour Biol. 18:278.
  4. Swissprot accession Q8WXI7.
  5. Kaneko, O. et al. (2009) J. Biol. Chem. 284:3739.
  6. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  7. Gubbels, J. A. A. et al. (2006) Mol. Cancer 5:50.
  8. Seelenmeyer, C. et al. (2003) J. Cell Sci. 116:1305.
  9. Patankar, M. S. et al. (2005) Gynecol. Oncol. 99:704.
  10. Belisle, J. A. et al. (2007) Immunology 122:418.
  11. Blalock, T. D. et al. (2007) Invest. Ophthalmol. Vis. Sci. 48:4509.

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Publications for CA125/MUC16 (5609-MU)(12)

We have publications tested in 5 confirmed species: Human, Mouse, Akkermansia muciniphila, Bacteria - E. Coli, N/A.

We have publications tested in 7 applications: Binding Assay, Bioassay, ELISA, ELISA (Standard), Reference Standard, Surface Plasmon Resonance, Western Blot.

Filter By Application
Binding Assay
ELISA (Standard)
Reference Standard
Surface Plasmon Resonance
Western Blot
All Applications
Filter By Species
Akkermansia muciniphila
Bacteria - E. Coli
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 5609-MU Applications Species
B Szymanska, Z Lukaszewsk, K Hermanowic, E Gorodkiewi A Multiple-Array SPRi Biosensor as a Tool for Detection of Gynecological-Oncological Diseases Biosensors, 2023-02-16;13(2):. 2023-02-16 [PMID: 36832045] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
LM Molinos-Al, V Lorin, V Monceaux, S Orr, A Essat, J Dufloo, O Schwartz, C Rouzioux, L Meyer, L Hocqueloux, A Sáez-Cirió, H Mouquet, ANRS VISCO Transient viral exposure drives functionally-coordinated humoral immune responses in HIV-1 post-treatment controllers Nature Communications, 2022-04-11;13(1):1944. 2022-04-11 [PMID: 35410989] (Bioassay, Human) Bioassay Human
DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences The Journal of Biological Chemistry, 2022-04-09;0(0):101917. 2022-04-09 [PMID: 35405095] (Bioassay, Akkermansia muciniphila) Bioassay Akkermansia muciniphila
J Song, LJ Sokoll, DW Chan, Z Zhang Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays Biomedicines, 2021-12-14;9(12):. 2021-12-14 [PMID: 34944713] (Reference Standard, Human) Reference Standard Human
PC Wu, CP Pai, MJ Lee, W Lee A Single-Substrate Biosensor with Spin-Coated Liquid Crystal Film for Simple, Sensitive and Label-Free Protein Detection Biosensors, 2021-10-06;11(10):. 2021-10-06 [PMID: 34677330] (ELISA, N/A) ELISA N/A
H Shaban, MJ Lee, W Lee Label-Free Detection and Spectrometrically Quantitative Analysis of the Cancer Biomarker CA125 Based on Lyotropic Chromonic Liquid Crystal Biosensors, 2021-08-11;11(8):. 2021-08-11 [PMID: 34436073] (Bioassay, Human) Bioassay Human
KA Malsagova, TO Pleshakova, RA Galiullin, AF Kozlov, ID Shumov, VP Popov, FV Tikhonenko, AV Glukhov, VS Ziborov, OF Petrov, VE Fortov, AI Archakov, YD Ivanov Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor Biosensors, 2020-12-18;10(12):. 2020-12-18 [PMID: 33353197] (Binding Assay, N/A) Binding Assay N/A
JK Fierle, J Abram-Sali, M Brioschi, M deTiani, G Coukos, SM Dunn Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery Sci Rep, 2019-09-06;9(1):12815. 2019-09-06 [PMID: 31492910] (Bioassay, Human) Bioassay Human
SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins Proc. Natl. Acad. Sci. U.S.A., 2019-03-25;0(0):. 2019-03-25 [PMID: 30910957] (Bioassay, Bacteria - E. Coli) Bioassay Bacteria - E. Coli
Establishment of patient-derived tumor xenograft models of epithelial ovarian cancer for pre-clinical evaluation of novel therapeutics Clin Cancer Res, 2016-08-29;0(0):. 2016-08-29 [PMID: 27573169] (ELISA (Standard), Mouse) ELISA (Standard) Mouse
Show All 12 Publications.

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Gene Symbol MUC16