NARFL Antibody - BSA Free

Immunohistochemistry: NARFL Antibody [NBP3-06258] - Immunochemical staining of human NARFL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Western Blot: NARFL Antibody [NBP3-06258] - Anti-NARFL rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:57 kDa

Immunohistochemistry-Paraffin: NARFL Antibody [NBP3-06258] - Immunochemical staining of human NARFL in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: NARFL Antibody [NBP3-06258] - NARFL was immunoprecipitated using:Lane A:0.5 mg HELA Whole Cell Lysate4 uL anti-NARFL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-NARFL rabbit polyclonal antibody, at 1:100 dilution.Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 56 kDaObserved band size :56 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NARFL Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human NARFL fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CIAO3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for NARFL Antibody - BSA Free

• cytosolic Fe-S cluster assembly factor NARFL
• FLJ21988
• HPRN
• IOP1Protein related to Narf
• Iron-only hydrogenase-like protein 1
• LET1 like/JFP15
• Nuclear prelamin A recognition factor-like protein
• nuclear prelamin A recognition factor-like
• PRNLET1L

Background

NARFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation ofextramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effectcould be indirect

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CIAO3