CARD14 Antibody - Azide and BSA Free

Western Blot: CARD14 Antibody [NBP2-92873] - Analysis of extracts of various cell lines, using CARD14 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CARD14 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human CARD14 (NP_001244899.1). SLSLHYSNALQEKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFSLAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNALQAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEARTREPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAEDFGEEPWSFS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD14
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 113 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for CARD14 Antibody - Azide and BSA Free

• bcl10-interacting maguk protein 2
• BIMP2
• CARD-containing MAGUK protein 2
• card-maguk protein 2
• Carma 2
• CARMA2CARD-containing MAGUK 2 protein
• caspase recruitment domain family, member 14
• caspase recruitment domain-containing protein 14

Background

The protein encoded by the CARD14 gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class ofproteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions ofthe plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying acharacteristic caspase-associated recruitment domain (CARD). This protein shares a similar domain structure withCARD11 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein knownto function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this proteinactivated NF-kappaB and induced the phosphorylation of BCL10. Two alternatively spliced variants of this gene encodingdistinct isoforms have been reported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: CARD14