Nanog Antibody


Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. more
Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were more
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog (NB100-58842) used at adilution of 1:250. Detection: DAB staining.
Western Blot: Nanog Antibody [NB100-58842] - Mouse Nanog.
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog used at a dilution of 1:500 (2ug/ml). Detection: DAB

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP
1 mg/ml

Order Details

Nanog Antibody Summary

A synthetic peptide made to the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64]
Embryonic Stem Cell Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This Nanog antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, and Immunohistochemistry on paraffin embedded tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Chromatin Immunoprecipitation and Flow Cytometry was reported in a scientific publication (PMID: 23735977).
Read Publications using
NB100-58842 in the following applications:

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Japanese house mouse.

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox


In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53. Nanog seems to be very important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors, indicating the need for further mechanistic exploration into its activity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
Species: Hu, Mu
Applications: WB, ICFlow
Species: Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC, ICFlow
Species: Hu, Mu, Rt
Applications: WB, IP, ICC, ICFlow
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP

Publications for Nanog Antibody (NB100-58842)(34)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, FLOW, ICC/IF, IHC, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-58842 Applications Species
Pandolfini L, Luzi E, Bressan D et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. May 7 2016 12:00AM [PMID:27154007] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Neri T, Muggeo S, Paulis M et al. Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts. Stem Cell Reports 2015 Sep 02 [PMID:26344905] (ICC/IF, Mouse) ICC/IF Mouse
Kim J, Liu Y, Qiu M, Xu Y. Pluripotency factor Nanog is tumorigenic by deregulating DNA damage response in somatic cells Oncogene. 2015 Jun 15 [PMID:26073077] (WB, IHC, Mouse) WB, IHC Mouse
Palla AR, Piazzolla D, Alcazar N et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015 May 20 [PMID:25988972] (IHC-P, Mouse) IHC-P Mouse
Palla AR, Piazzolla D, Alcazar N et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep 2015 [PMID:25988972] (IHC, Mouse) IHC Mouse
Bertacchi M, Pandolfini L, D'Onofrio M et al. The double inhibition of endogenously-produced BMP and Wnt factors synergistically triggers dorsal telencephalic differentiation of mouse ES cells. Dev Neurobiol 2014 Jul 15 [PMID:25044881] (ICC/IF, Mouse) ICC/IF Mouse
Carelli S, Messaggio F, Canazza A et al. Characteristics and Properties of Mesenchymal Stem Cells Derived from Micro-fragmented Adipose Tissue. Cell Transplant. 2014 May 6 [PMID:24806078] (IHC-P, Human) IHC-P Human
Vaz MA, Martinez JC, Devesa JM et al. Prognostic Value of Stem Cell Quantification in Stage II Colon Cancer. PLoS ONE 2014 Mar 3 [PMID:24586330] (IHC-P, Human) IHC-P Human
Abad M, Mosteiro L, Pantoja C et al. Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Nature. 2013 Oct 17 [PMID:24025773] (ICC/IF, Mouse) ICC/IF Mouse
Schneider RP, Garrobo I, Foronda M et al. TRF1 is a stem cell marker and is essential for the generation of induced pluripotent stem cells. Nat Commun 2013 Jun 5 [PMID:23735977] (FLOW, ChIP, Mouse) FLOW, ChIP Mouse
Show All 34 Publications.

Reviews for Nanog Antibody (NB100-58842) (0)

There are no reviews for Nanog Antibody (NB100-58842). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Nanog Antibody (NB100-58842) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Nanog Antibody Products

Related Products by Gene

Bioinformatics Tool for Nanog Antibody (NB100-58842)

Discover related pathways, diseases and genes to Nanog Antibody (NB100-58842). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nanog Antibody (NB100-58842)

Discover more about diseases related to Nanog Antibody (NB100-58842).

Pathways for Nanog Antibody (NB100-58842)

View related products by pathway.

PTMs for Nanog Antibody (NB100-58842)

Learn more about PTMs related to Nanog Antibody (NB100-58842).

Research Areas for Nanog Antibody (NB100-58842)

Find related products by research area.

Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NANOG

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-58842 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought