Nanog Antibody

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Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. ...read more
Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog (NB100-58842) used at adilution of 1:250. Detection: DAB staining.
Western Blot: Nanog Antibody [NB100-58842] - Mouse Nanog.
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog used at a dilution of 1:500 (2ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Nanog Antibody Summary

Immunogen
A synthetic peptide made to the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64]
Localization
Nucleus.
Marker
Embryonic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NANOG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 - 5 ug/mg lysate
Application Notes
This Nanog antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, and Immunohistochemistry on paraffin embedded tissue. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA (pH 9.0) is recommended. Use in Chromatin Immunoprecipitation and Flow Cytometry was reported in a scientific publication (PMID: 23735977).
Publications
Read Publications using
NB100-58842 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53. Nanog seems to be very important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors, indicating the need for further mechanistic exploration into its activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nanog Antibody (NB100-58842)(35)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, FLOW, ICC/IF, IHC, IHC-P, WB.


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ChIP
(1)
FLOW
(1)
ICC/IF
(10)
IHC
(2)
IHC-P
(4)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB100-58842 Applications Species
Zhang N, Lyu Y, Pan X et al. miR-146b-5p promotes the neural conversion of pluripotent stem cells by targeting Smad4 Int. J. Mol. Med. 2017 Jul 11 [PMID: 28713933] (ICC/IF, Mouse) ICC/IF Mouse
Pandolfini L, Luzi E, Bressan D et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. May 7 2016 [PMID: 27154007] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Paulis M, Castelli A, Susani L et al. Chromosome transplantation as a novel approach for correcting complex genomic disorders. Oncotarget 2015 Nov 3 [PMID: 26485770] (ICC/IF) ICC/IF
Neri T, Muggeo S, Paulis M et al. Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts. Stem Cell Reports 2015 Sep 02 [PMID: 26344905] (ICC/IF, Mouse) ICC/IF Mouse
Kim J, Liu Y, Qiu M, Xu Y. Pluripotency factor Nanog is tumorigenic by deregulating DNA damage response in somatic cells Oncogene. 2015 Jun 15 [PMID: 26073077] (WB, IHC, Mouse) WB, IHC Mouse
Palla AR, Piazzolla D, Alcazar N et al. The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep. 2015 May 20 [PMID: 25988972] (IHC-P, Mouse)

Details:
Nanog antibody was used for IHC-P application on sections of tail skin from tissue specific doxycycline-inducible Nanog transgenic/TG mouse model (K5-rt-TA^tg/.;Col1a1^tetO-Nanog/?+?) and their control/CTR littermates lacking the rtTA transactivator (Col1a1^tetO-Nanog/?+) treated with 2?mg/ml of doxycycline/DOX for 48?hours (Figure 1C), and on CTR / TG papillomas (Figure S2E, Figure S3A). IHC-P was also performed on tail skin of TPA-treated CTR and TG mice (Figure 1F), and on SCC and lymph node metastasis from the same mouse (Figure S2F).
IHC-P Mouse
Bertacchi M, Pandolfini L, D'Onofrio M et al. The double inhibition of endogenously-produced BMP and Wnt factors synergistically triggers dorsal telencephalic differentiation of mouse ES cells. Dev Neurobiol 2014 Jul 15 [PMID: 25044881] (ICC/IF, Mouse)

Details:
Nanog antibody used for ICC-IF on undifferentiated mouse ES cells (E14 Tg2A ES cell line) alongwith another pluripotency marker (Oct4) at Day 0 and 2 of in-vitro differentiation (Supporting Information Fig. 1A) - Cells grown on Poly-ornithine/Laminin coated glass coverslips, fixed with 2% paraformaldehyde for 10-15 min, permeabilized using 0.1% Triton X100 in PBS and blocked with 0.5% BSA in PBS, primary used at 1:300 dilution - incubated 2 h RT.
ICC/IF Mouse
Carelli S, Messaggio F, Canazza A et al. Characteristics and Properties of Mesenchymal Stem Cells Derived from Micro-fragmented Adipose Tissue. Cell Transplant. 2014 May 6 [PMID: 24806078] (IHC-P, Human) IHC-P Human
Vaz MA, Martinez JC, Devesa JM et al. Prognostic Value of Stem Cell Quantification in Stage II Colon Cancer. PLoS ONE 2014 Mar 3 [PMID: 24586330] (IHC-P, Human) IHC-P Human
Abad M, Mosteiro L, Pantoja C et al. Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Nature. 2013 Oct 17 [PMID: 24025773] (ICC/IF, Mouse) ICC/IF Mouse
Show All 35 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot