Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. ...read more
Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog (NB100-58842) used at adilution of 1:250. Detection: DAB staining.
Western Blot: Nanog Antibody [NB100-58842] - Mouse Nanog.
Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Nanog used at a dilution of 1:500 (2ug/ml). Detection: DAB
This Nanog antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, and Immunohistochemistry on paraffin embedded tissue. Prior to immunostaining paraffin tissues, antigen retrieval with Tris-EDTA (pH 9.0) is recommended. Use in Chromatin Immunoprecipitation and Flow Cytometry was reported in a scientific publication (PMID: 23735977).
Read Publications using NB100-58842 in the following applications:
In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53. Nanog seems to be very important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors, indicating the need for further mechanistic exploration into its activity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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