Nanog Antibody


Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.
Flow Cytometry: Nanog Antibody [NB100-588] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Flow Cytometry: Nanog Antibody [NB100-588] - staining of NTERA-2 cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP
1.0 mg/ml

Order Details

Nanog Antibody Summary

A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).
Embryonic Stem Cell Marker
NB100-588 is specific for the Nanog protein.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Reviewed Applications
Read 3 Reviews rated 4
NB100-588 in the following application:

Read Publications using
NB100-588 in the following applications:

  • 2 publications
  • WB
    4 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Southeastern Asian house mouse, Western Mediterranean mouse and Japanese house mouse.

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox


Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rb(-)
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Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP

Publications for Nanog Antibody (NB100-588)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.

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Showing Publications 1 - 9 of 9.
Publications using NB100-588 Applications Species
Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016 Aug 31 [PMID:27590511] (WB, Human) WB Human
Zhang C, Samanta D, Lu H et al. Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proc Natl Acad Sci U S A. 2016 Mar 21 [PMID:27001847] (WB, Human) WB Human
Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015 Jul 30 [PMID:26229077] (WB, Human) WB Human
Hamada M, Malureanu LA, Wijshake T et al. Reprogramming to pluripotency can conceal somatic cell chromosomal instability. PLoS Genet 2012 [PMID:22952451]
Wu Y, Guo Z, Wu H et al. SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2. PLoS One 2012 [PMID:22745796]
Ganuza M, Saiz-Ladera C, Canamero M, Gomez G, Schneider R, Blasco MA, Pisano D, Paramio JM, Santamaria D, Barbacid M. Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion. EMBO J. 2012 Apr 13. [PMID:22505032] (ICC/IF, Mouse) ICC/IF Mouse
Morgado-Palacin L, Llanos S, Serrano M Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells. Cell Cycle. 2012 Feb 1 [PMID:22262176] (ICC/IF, Mouse) ICC/IF Mouse
Kohler EE, Cowan CE, Chatterjee I et al. NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis Blood 2011 Feb 3 [PMID:21119109] (WB, Human) WB Human
Carey BW, Markoulaki S, Hanna J et al. Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A 2009 Jan [PMID:19109433]

Reviews for Nanog Antibody (NB100-588) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2 application: WB, Flow.

Reviews using NB100-588:
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Images Ratings Applications Species Date Details
Western Blot Nanog NB100-588
reviewed by:
WB Human 08/22/2015


ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate


Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000


Detection NotesRegular Western blot Protocol
Flow Cytometry Nanog NB100-588
reviewed by:
Flow Mouse 04/29/2014


ApplicationFlow Cytometry
Western Blot Nanog NB100-588
reviewed by:
WB Human 06/08/2011


ApplicationWestern Blot
Sample TestedHUVEC, MCF7, JURKAT, Sample Amount: 2-30 ug
Pub Med ID21119109


Blocking DetailsBlocking Buffer: 5% milk in TBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 8 ug/ml, Incubation Dilution Buffer: 3% BSA in TBS, Incubation Time: overnight/2 hours, Incubation Temp: 4/25C

Secondary Antibody

Secondary DescriptionSecondary Ab: diluted in 5% milk in TBS, Secondary Ab Dilution Ratio: 1:1000


Detection NotesDetection Method: Chemiluminescence, Exposure Time: 3 minutes, Specific Bands: 35 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nanog Antibody (NB100-588) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for Nanog Antibody (NB100-588)

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Research Areas for Nanog Antibody (NB100-588)

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NANOG

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