Nanog Antibody

Images

 
Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.
Flow Cytometry: Nanog Antibody [NB100-588] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Flow Cytometry: Nanog Antibody [NB100-588] - staining of NTERA-2 cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Nanog Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).
Marker
Embryonic Stem Cell Marker
Specificity
NB100-588 is specific for the Nanog protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NANOG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:150
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 3 Reviews rated 4
using
NB100-588 in the following applications:

Publications
Read Publications using
NB100-588 in the following applications:

  • 2 publications
  • WB
    5 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Southeastern Asian house mouse, Western Mediterranean mouse and Japanese house mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
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Publications for Nanog Antibody (NB100-588)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-588 Applications Species
Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment. Cell Rep. [PMID: 28228260] (WB, Human) WB Human
Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016 Aug 31 [PMID: 27590511] (WB, Human) WB Human
Zhang C, Samanta D, Lu H et al. Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proc Natl Acad Sci U S A. 2016 Mar 21 [PMID: 27001847] (WB, Human) WB Human
Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015 Jul 30 [PMID: 26229077] (WB, Human) WB Human
Hamada M, Malureanu LA, Wijshake T et al. Reprogramming to pluripotency can conceal somatic cell chromosomal instability. PLoS Genet 2012 [PMID: 22952451]
Wu Y, Guo Z, Wu H et al. SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2. PLoS One 2012 [PMID: 22745796]
Ganuza M, Saiz-Ladera C, Canamero M, Gomez G, Schneider R, Blasco MA, Pisano D, Paramio JM, Santamaria D, Barbacid M. Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion. EMBO J. 2012 Apr 13. [PMID: 22505032] (ICC/IF, Mouse) ICC/IF Mouse
Morgado-Palacin L, Llanos S, Serrano M Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells. Cell Cycle. 2012 Feb 1 [PMID: 22262176] (ICC/IF, Mouse) ICC/IF Mouse
Kohler EE, Cowan CE, Chatterjee I et al. NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis Blood 2011 Feb 3 [PMID: 21119109] (WB, Human) WB Human
Carey BW, Markoulaki S, Hanna J et al. Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A 2009 Jan [PMID: 19109433]

Reviews for Nanog Antibody (NB100-588) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 2 application: WB, Flow.

Reviews using NB100-588:
Filter by Applications
WB
(2)
Flow
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Nanog NB100-588
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reviewed by:
Anonymous
WB Human 08/22/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol
Flow Cytometry Nanog NB100-588
Enlarge
reviewed by:
Anonymous
Flow Mouse 04/29/2014
View

Summary

ApplicationFlow Cytometry
SpeciesMouse
Western Blot Nanog NB100-588
Enlarge
reviewed by:
Anonymous
WB Human 06/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedHUVEC, MCF7, JURKAT, Sample Amount: 2-30 ug
SpeciesHuman
Pub Med ID21119109

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 8 ug/ml, Incubation Dilution Buffer: 3% BSA in TBS, Incubation Time: overnight/2 hours, Incubation Temp: 4/25C

Secondary Antibody

Secondary DescriptionSecondary Ab: diluted in 5% milk in TBS, Secondary Ab Dilution Ratio: 1:1000

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 3 minutes, Specific Bands: 35 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nanog Antibody (NB100-588) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nanog Antibody (NB100-588)

Discover related pathways, diseases and genes to Nanog Antibody (NB100-588). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nanog Antibody (NB100-588)

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Pathways for Nanog Antibody (NB100-588)

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Research Areas for Nanog Antibody (NB100-588)

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

Anonymous
08/22/2015
Application: WB
Species: Human

Anonymous
04/29/2014
Application: Flow
Species: Mouse

Anonymous
06/08/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot

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