NALP12 Antibody - BSA Free

Images

 
Western Blot: NALP12 Antibody [NBP1-76293] - B6 mice (n = 6) were infected ocularly with 2.5 x 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 and KOS63 (mock-infected ...read more
Immunocytochemistry/ Immunofluorescence: NALP12 Antibody [NBP1-76293] - Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-76293] - Human brain tissue. Dilution: 5 ug/mL
Western Blot: NALP12 Antibody [NBP1-76293] - Human brain tissue lysate with NALP12 antibody at 1 ug/mL.
Biological Strategies: Western Blot: NALP12 Antibody [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, V-ViSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

NALP12 Antibody - BSA Free Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP12. The immunogen is located within amino acids 110 - 160 of NALP12. Amino Acid Squence: TPRKDPQETYRDYVRRKF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP12
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 ug/ml
Control Peptide
NALP12 Peptide (NBP1-76293PEP)
Publications
Read Publication using
NBP1-76293 in the following applications:

  • WB
    1 publication

Reactivity Notes

Viral reactivity reported in scientific literature (PMID: 31367214).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for NALP12 Antibody - BSA Free

  • FCAS2
  • Monarch-1
  • NACHT, leucine rich repeat and PYD containing 12
  • NACHT, LRR and PYD domains-containing protein 12
  • NALP12LRR and PYD containing protein 12
  • NLR family, pyrin domain containing 12
  • nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 12
  • PYPAF7Monarch1
  • PYRIN-containing APAF1-like protein 7
  • Regulated by nitric oxide
  • RNO2

Background

NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-kappaB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NALP12 Antibody (NBP1-76293)(1)

We have publications tested in 1 confirmed species: Viral.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NALP12 Antibody (NBP1-76293). (Showing 1 - 1 of 1 FAQ).

  1. Actually i'm testing the mouse monoclonal anti-hNLRP12, but as in the image that you show for the rabbit policlonal anti-hNLRP12, i just see a band in the 95 kDa region, so i would like to know why the antibody doesn't recognize a band at aprox 120 kDa, do you know about a postranscriptional modification of this protein??
    • There are several different characterized isoform of NALP12. Please visit the following website to learn more, UniProt P59046. The band that you see in our western blot reflects the NALP12 isoform expressed in the tissue probed, and it may vary between different tissues and species.

Secondary Antibodies

 

Isotype Controls

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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

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Bioinformatics

Gene Symbol NLRP12
Uniprot