NALP12 Antibody - BSA Free

Western Blot: NALP12 Antibody [NBP1-76293] - B6 mice (n = 6) were infected ocularly with 2.5 x 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 and KOS63 (mock-infected mice (n = 6) used as controls). 48 hrs later, animals were euthanized & corneas were excised & homogenized in lysis buffer, and WB was performed for NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 (beta-actin used as a control). Three WB performed using different corneal lysates harvested from three sets of corneas from 2 mice (4 corneas/lysate). Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, and AIM2 at 48 hrs post-infection compared to controls, and normalized to beta-actin. Representative densitometric analysis is shown compared to control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fimmu.2019.01631/full), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: NALP12 Antibody [NBP1-76293] - Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-76293] - Human brain tissue. Dilution: 5 ug/mL

Western Blot: NALP12 Antibody [NBP1-76293] - Human brain tissue lysate with NALP12 antibody at 1 ug/mL.

Western Blot: NALP12 Antibody [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with clinical isolates KOS79 and KOS63 at MOI of 1. Two, 8 and 24 h post-infection, hTCEpi cells were harvested and immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes. Corresponding b-actin was used as a control. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2019.01631/full), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, V-Vi
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NALP12 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP12. The immunogen is located within amino acids 110 - 160 of NALP12. Amino Acid Squence: TPRKDPQETYRDYVRRKF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP12
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 ug/ml
• Control Peptide:

  NALP12 Peptide (NBP1-76293PEP)

Reactivity Notes

Viral reactivity reported in scientific literature (PMID: 31367214).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for NALP12 Antibody - BSA Free

• FCAS2
• Monarch-1
• NACHT, leucine rich repeat and PYD containing 12
• NACHT, LRR and PYD domains-containing protein 12
• NALP12LRR and PYD containing protein 12
• NLR family, pyrin domain containing 12
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 12
• PYPAF7Monarch1
• PYRIN-containing APAF1-like protein 7
• Regulated by nitric oxide
• RNO2

Background

NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-kappaB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NALP12 Antibody (NBP1-76293)(1)

Publication using NBP1-76293

• Coulon PG, Dhanushkodi N, Prakash S et al. NLRP3, NLRP12, and IFI16 Inflammasomes Induction and Caspase-1 Activation Triggered by Virulent HSV-1 Strains Are Associated With Severe Corneal Inflammatory Herpetic Disease Front Immunol Jul 16 2019 [PMID: 31367214] (WB, Viral)

FAQs for NALP12 Antibody (NBP1-76293). (Showing 1 - 1 of 1 FAQ).

1. Actually i'm testing the mouse monoclonal anti-hNLRP12, but as in the image that you show for the rabbit policlonal anti-hNLRP12, i just see a band in the 95 kDa region, so i would like to know why the antibody doesn't recognize a band at aprox 120 kDa, do you know about a postranscriptional modification of this protein??
   • There are several different characterized isoform of NALP12. Please visit the following website to learn more, UniProt P59046. The band that you see in our western blot reflects the NALP12 isoform expressed in the tissue probed, and it may vary between different tissues and species.

Bioinformatics

• Gene Symbol: NLRP12
• Uniprot:
  • NP_653288(Human)