NAC1 Antibody [Unconjugated]

Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAC1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NAC1 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Sheep Anti-Human NAC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8375) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NAC1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAC1 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

NAC1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NAC1
  Val471-Gln527
  Accession # Q96RE7
• Specificity: Detects human NAC1 in direct ELISAs and Western Blots. In direct ELISAs, appoximately 100% cross-reactivity with recombinant mouse NAC1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NACC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NAC1 Antibody [Unconjugated]

• BEND8
• BTB (POZ) domain containing 14B
• BTB/POZ domain-containing protein 14B
• BTBD14B
• BTBD30
• NAC1
• NAC1BEN domain containing 8
• NAC-1FLJ37383
• NACC1
• nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• nucleus accumbens-associated protein 1
• transcriptional repressor NAC1

Background

NAC1 (nucleus accumbens-associated protein 1; also NACC1) is a 527 aa BTB/POZ domain containing protein. Human NAC1 shares 86% and 85% aa identity with mouse and rat NAC1, respectively.  It has been shown to have a role in transcriptional regulation in both pluripotent stem cells and in the brain.  Additionally, NAC1 expression is upregulated in many cancers and this expression has been suggested to correlate with increased aggressiveness in some cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NACC1
• Uniprot:
  • Q96RE7()