NAC1 Antibody (PCRP-NACC1-1A8) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: Flow, MA
• Clone: PCRP-NACC1-1A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

NAC1 Antibody (PCRP-NACC1-1A8) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Recombinant full-length human NAC1 protein (Uniprot: Q96RE7)
• Localization: Nucleus, Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NACC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Protein Array
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NAC1 Antibody (PCRP-NACC1-1A8) [CoraFluor™ 1]

• BEND8
• BTB (POZ) domain containing 14B
• BTB/POZ domain-containing protein 14B
• BTBD14B
• BTBD30
• NAC1
• NAC1BEN domain containing 8
• NAC-1FLJ37383
• NACC1
• nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• nucleus accumbens-associated protein 1
• transcriptional repressor NAC1

Background

The BTB/POZ gene family shares a conserved BTB/POZ protein-protein interaction motif at the N terminus that mediates homodimer or heterodimer formation. NAC1, a member of this family, has been found to be overexpressed in ovarian cancer. This protein appears to play a critical role in tumorigenesis and in the growth and survival of tumor cells, and Nac1 antibodies are therefore useful for cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NACC1