Nab2 Antibody - BSA Free

Western Blot: Nab2 Antibody [NBP1-71869] - Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-NAB2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB2 was also immunoprecipitated by another rabbit anti-NAB2 antibody which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Nab2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Nab2 Antibody - BSA Free (NBP1-71869) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 475 and 525 of human NGFI-A Binding Protein 2 using the numbering given in entry NP_005958.1 (GeneID 4665).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nab2 Antibody - BSA Free

• EGR-1-binding protein 2
• MADEREGR1 binding protein 2
• Melanoma-associated delayed early response protein
• MGC75085
• NGFI-A binding protein 2 (EGR1 binding protein 2)
• NGFI-A-binding protein 2
• Protein MADER

Background

This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAB2
• Entrez:
  • 4665(Human)
• Uniprot:
  • Q15742(Human)