MYH6 Antibody (CL2162) - BSA Free

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746] - Staining in human heart muscle and liver tissues. Corresponding MYH6 RNA-seq data are presented for the same tissues.

Western Blot: MYH6 Antibody (CL2162) [NBP2-36746] - Lane 1: Marker [kDa] Lane 2: Human skeletal muscle tissue lysate

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746] - Staining of human heart muscle shows very strong cytoplasmic positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746] - Staining of human skeletal muscle shows strong cytoplasmic positivity in striated muscle fibers.

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL2162
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MYH6 Antibody (CL2162) - BSA Free Summary

• Description: Novus Biologicals Mouse MYH6 Antibody (CL2162) - BSA Free (NBP2-36746) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P13533, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MYH6 Antibody (CL2162) - BSA Free

• alpha-MHC
• ASD3
• cardiomyopathy
• CMD1EE
• CMH14
• Hypertrophic 1
• MYH6
• MYHC
• MYHCA
• MyHC-Alpha
• Myosin Heavy Chain 6
• Myosin Heavy Chain, Cardiac Muscle Alpha Isoform
• Myosin, Heavy Chain 6, Cardiac Muscle, Alpha
• Myosin, Heavy Polypeptide 6, Cardiac Muscle, Alpha (Cardiomyopathy, Hypertrophic 1)
• Myosin-6
• SSS3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for MYH6 Antibody (NBP2-36746) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC Human 06/29/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Formalin Fixed Paraffin Embedded Heart
• Species: Human

Comments

• Comments: WD [1:4000], HIER required with Citrate Buffer pH 6.0 10mM; secondary Ab RTU Polymer-HRP

reviewed by: Verified Customer WB Human 06/22/2015

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: MYH6
• Uniprot:
  • P13533()