Myosin heavy chain 2 Antibody - BSA Free

Western Blot: Myosin heavy chain 2 Antibody [NBP2-94624] - Analysis of extracts of Mouse skeletal muscle cells, using MYH2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: Myosin heavy chain 2 Antibody [NBP2-94624] - Analysis of extracts of Rat skeletal muscle cells, using MYH2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.

Myosin heavy chain 2 Antibody [NBP2-94624] -Analysis of mouse skeletal muscle cells using MYH2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Myosin heavy chain 2 Antibody [NBP2-94624] -Analysis of rat skeletal muscle cells using MYH2 Rabbit pAb (A15292) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] - Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using Myosin heavy chain 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] - Western blot analysis of lysates from U-2 OS cells using Myosin heavy chain 2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 60 s.

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 2 Antibody - Azide and BSA Free [Myosin heavy chain 2] - Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Myosin heavy chain 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Myosin heavy chain 2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Myosin heavy chain 2 (NP_001093582.1). AQLFSGAQTAEGEGA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 223 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Myosin heavy chain 2 Antibody - BSA Free

• fast 2a myosin heavy chain
• MYH2
• MYH2A
• MYHas8
• MyHC-2a
• MyHC-IIa
• MYHSA2
• Myosin heavy chain 2
• myosin heavy chain 2a
• myosin heavy chain IIa
• myosin, heavy chain 2, skeletal muscle, adult
• myosin, heavy polypeptide 2, skeletal muscle, adult
• myosin-2
• skeletal muscle, adult 2
• type IIA myosin heavy chain

Background

Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: MYH2