MYH7 Antibody - BSA Free

Western Blot: MYH7 Antibody - Azide and BSA Free [MYH7] - Western blot analysis of various lysates using MYH7 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 180s.

Immunocytochemistry/Immunofluorescence: MYH7 Antibody [NBP2-94079] - Analysis of RD cells using MYH7/beta-MHC Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MYH7 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1700-1800 of human MYH7 (NP_000248.2). KKMDADLSQLQTEVEEA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:100 - 1:500
• Theoretical MW: 223 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for MYH7 Antibody - BSA Free

• cardiac muscle beta isoform
• CMD1S
• CMD1SMGC138376
• CMH1
• DKFZp451F047
• MGC138378
• MPD1
• MYH7
• MYHCB
• MyHC-beta
• myhc-slow
• myopathy, distal 1
• myosin heavy chain (AA 1-96)
• myosin, heavy chain 7, cardiac muscle, beta
• myosin, heavy polypeptide 7, cardiac muscle, beta
• Myosin-7
• rhabdomyosarcoma antigen MU-RMS-40.7A
• SPMD
• SPMM

Background

MYH7, also known as Myosin-7, is a 1,935 amino acid protein that is 223 kDa, found predominantly in myocytes and mediates plus-ended movement along microfilaments, it is involved in muscle contraction through cyclic interactions with actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations. Disease research is currently being studied with relation to this protein and myopathy, hypertrophic cardiomyopathy, familial hypertrophic cardiomyopathy, dilated cardiomyopathy, wolff-parkinson-white syndrome, left ventricular noncompaction, left ventricular noncompaction 5, long qt syndrome, endocardial fibroelastosis, scapuloperoneal syndrome, laing distal myopathy, rhabdomyosarcoma oculopharyngeal muscular dystrophy, ebstein anomaly, acute myocardial infarction, muscular dystrophy, myotonic dystrophy, and myocardial infarction. Its involvement has been observed with relation to YWHAQ, MYH13, MYL3, YWHAZ, MYH9, ACTB, NTHL1, and over 100 other proteins in cell adhesion integrin-mediated cell adhesion and migration, cell adhesion tight junctions, cytoskeleton remodeling regulation of actin cytoskeleton by Rho GTPases, immune response CCR3 signaling in eosinophils, development MAG-dependent inhibition of neurite outgrowth, RhoA pathway, factors promoting cardiogenesis in vertebrates, antioxidant action of vitamin-C, transendothelial migration of leukocytes, actin nucleation by ARP-WASP complex, cardiac muscle contraction, hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy, and viral myocarditis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYH7 Antibody (NBP2-94079)(1)

Publication using NBP2-94079

• Johnson LL, Hebert S, Kueppers RB, McLoon LK Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles Investigative ophthalmology & visual science 2023-09-01 [PMID: 37703038] (IHC-Fr, Mouse)
  
  Details:
  Dilution: 1:50

Bioinformatics

• Gene Symbol: MYH7